Flow Cytometry Shared Resource The Flow Cytometry Shared Resource has been an integral component of the UC San Diego Moores Cancer Center since 1990. It exists to provide peer-review, funded investigators from all Divisions of the Cancer Center, as well other investigators from across and the La Jolla biomedical research community, with ready access to high-speed analysis and/or cell sorting of dissociated cell populations from clinical samples, animal experiments and cell culture studies. The services available from this Resource are as follows: Analytical Flow Cytometry: can be performed on FACSCalibu, FACSAria I or FACSAria II flow cytometers depending on the application. The FACSCalibur is a bench-top flow cytometer that can be used for analysis of up to four-colors of fluorescence and two independent light scatter parameters. The FACSAria is a three-laser flow cytometer that can be used for analyses of cells/particles labeled with up to eleven colors of fluorescence, along with forward and side-angle light scatter parameters. The FACSAria utilizes FACSDiva software, which has many advanced features, such as exponential scaling, adaptive gating, and automated fluorescence spectral compensation. The FACSCalibur flow cytometer computer system uses CELLQuest software for list-mode data recording and analysis. These services allow investigators to analyze cell populations for hundreds of different types of experiments, including cell cycle status, surface and/or cytoplasmic antigen phenotype, apoptosis, or expression of a transgene (e.g. following transduction with green fluorescent protein [GFP]).

Public Health Relevance

The availability of the Flow Cytometry Shared Resource enables our researchers to conduct high-speed analysis and/or cell sorting of dissociated cell populations from clinical samples, animal experiments and cell culture studies.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Shafik, Hasnaa
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University of California San Diego
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Sen, Supriya; Langiewicz, Magda; Jumaa, Hassan et al. (2015) Deletion of serine/arginine-rich splicing factor 3 in hepatocytes predisposes to hepatocellular carcinoma in mice. Hepatology 61:171-83
Hoffmann, Hanne M; Tamrazian, Anika; Xie, Huimin et al. (2014) Heterozygous deletion of ventral anterior homeobox (vax1) causes subfertility in mice. Endocrinology 155:4043-53
Hartman, Lisa L R; Crawford, John R; Makale, Milan T et al. (2014) Pediatric phase II trials of poly-ICLC in the management of newly diagnosed and recurrent brain tumors. J Pediatr Hematol Oncol 36:451-7
Williams, Richard T; Barnhill, Lisa M; Kuo, Huan-Hsien et al. (2014) Chimeras of p14ARF and p16: functional hybrids with the ability to arrest growth. PLoS One 9:e88219
Huang, Carlos P; Fofana, Mariama; Chan, Jefferson et al. (2014) Copper transporter 2 regulates intracellular copper and sensitivity to cisplatin. Metallomics 6:654-61
Paolini, Paul; Pick, Daniel; Lapira, Jennifer et al. (2014) Developmental and extracellular matrix-remodeling processes in rosiglitazone-exposed neonatal rat cardiomyocytes. Pharmacogenomics 15:759-74
Goellner, Eva M; Smith, Catherine E; Campbell, Christopher S et al. (2014) PCNA and Msh2-Msh6 activate an Mlh1-Pms1 endonuclease pathway required for Exo1-independent mismatch repair. Mol Cell 55:291-304
Glidewell-Kenney, Christine A; Trang, Crystal; Shao, Paul P et al. (2014) Neurokinin B induces c-fos transcription via protein kinase C and activation of serum response factor and Elk-1 in immortalized GnRH neurons. Endocrinology 155:3909-19
Jiang, P; Mukthavaram, R; Chao, Y et al. (2014) In vitro and in vivo anticancer effects of mevalonate pathway modulation on human cancer cells. Br J Cancer 111:1562-71
Jacques, Bonnie E; Montgomery 4th, William H; Uribe, Phillip M et al. (2014) The role of Wnt/*-catenin signaling in proliferation and regeneration of the developing basilar papilla and lateral line. Dev Neurobiol 74:438-56

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