Transgenic & Genetic Constructs (TGC) The Transgenic & Genetic Constructs (TGC) Shared Resource, directed by Dr. Steven Fiering, provides services for generating, maintaining, and genetically and experimentally manipulating genetically modified mice (GMM). TGC provides mouse, cell, and genetic construct manipulation and speed congenics to NCCC researchers. These technical services are provided by 3 technical specialists, one for the mouse work, one in Embryonic Stem cell culture and general molecular biology research, and the third performing automated SNP genotyping assays using Illumina(R) technology. Newer services of TGC involve using techniques to generate mice with either humanized immune systems or humanized livers. A colony of immunodeficient Nod/SCID/IL2gamma receptor knockout mice (NSG) is in high demand by NCCC researchers, because they are the optimal strain for establishing xenografts of human tumor cell lines. For many GMM-based experiments, knowing the genetic background carrying the genetic modifications is crucial. Traditionally, a 3-year process of random backcrossing has been required to change the genetic background of a GMM. Dr. James Gorham, with assistance from Fiering, established a speed congenic facility that uses automated SNP genotyping to perform speed congenic and related services on GMM. Merging TGC with the Speed Congenics Resource expanded TGC services to provide rapid identification of male breeders with the highest proportion of the desired background; this has cut the required number of backcross generations from 10 to 5. On a daily basis, Fiering focuses primarily on the transgenic mouse generation and utilization services, and Gorham focuses on the speed congenics, but they work together to direct TGC as one unit. Combining these services into TGC has enabled us to expand and improve services and efficiency to better serve our clients. The generation and utilization of GMM is a technically complex field, with new techniques rapidly evolving. TGC constantly is developing new technical capabilities and new services to broaden our ability to support GMM usage in cancer research. The vast majority of services provided by TGC utilize equipment (e.g., tissue culture hoods, molecular biology equipment, microscopes, and injection devices and surgical tools) that have been in the lab for most of the 16 years of its existence. Biological systems in use at a given time, technological approaches and the faculty and their interests constantly evolve. Our ability to provide the broad range of services is dependent primarily on the accumulated skill of the personnel performing the service tasks, such as culturing ES cells, manipulating embryos, using recombineering to generate constructs, and speed congenic analysis. TGC services are an important component of the research conducted by 4 of 6 NCCC Research Programs (Cancer Mechanisms, Molecular Therapeutics, Cancer Imaging & Radiobiology, and Immunology & Cancer Immunotherapy). TGC also has entered into shared service agreements with the University of Vermont Transgenic Shared Resource and Jackson Labs to avoid overlapping services and to refer clients for services not available at their institution.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee I - Career Development (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Dartmouth College
United States
Zip Code
Emond, Jennifer A; Tovar, Alison; Li, Zhigang et al. (2017) FTO genotype and weight status among preadolescents: Assessing the mediating effects of obesogenic appetitive traits. Appetite 117:321-329
Rapuano, Kristina M; Zieselman, Amanda L; Kelley, William M et al. (2017) Genetic risk for obesity predicts nucleus accumbens size and responsivity to real-world food cues. Proc Natl Acad Sci U S A 114:160-165
Carroll, A M; Cheng, R; Collie-Duguid, E S R et al. (2017) Fine-mapping of genes determining extrafusal fiber properties in murine soleus muscle. Physiol Genomics 49:141-150
Fang, Jun; Jia, Jinping; Makowski, Matthew et al. (2017) Functional characterization of a multi-cancer risk locus on chr5p15.33 reveals regulation of TERT by ZNF148. Nat Commun 8:15034
Pan, Yongchu; Liu, Hongliang; Wang, Yanru et al. (2017) Associations between genetic variants in mRNA splicing-related genes and risk of lung cancer: a pathway-based analysis from published GWASs. Sci Rep 7:44634
Rothwell, Simon; Cooper, Robert G; Lundberg, Ingrid E et al. (2017) Immune-Array Analysis in Sporadic Inclusion Body Myositis Reveals HLA-DRB1 Amino Acid Heterogeneity Across the Myositis Spectrum. Arthritis Rheumatol 69:1090-1099
Hampsch, Riley A; Shee, Kevin; Bates, Darcy et al. (2017) Therapeutic sensitivity to Rac GTPase inhibition requires consequential suppression of mTORC1, AKT, and MEK signaling in breast cancer. Oncotarget 8:21806-21817
Barr, Paul J; Forcino, Rachel C; Thompson, Rachel et al. (2017) Evaluating CollaboRATE in a clinical setting: analysis of mode effects on scores, response rates and costs of data collection. BMJ Open 7:e014681
Melin, Beatrice S; Barnholtz-Sloan, Jill S; Wrensch, Margaret R et al. (2017) Genome-wide association study of glioma subtypes identifies specific differences in genetic susceptibility to glioblastoma and non-glioblastoma tumors. Nat Genet 49:789-794
Adachi-Mejia, Anna M; Lee, Chanam; Lee, Chunkuen et al. (2017) Geographic variation in the relationship between body mass index and the built environment. Prev Med 100:33-40

Showing the most recent 10 out of 1659 publications