The ability to introduce foreign genes into the mammalian germ line, or to selectively ablate endogenous genes from the mouse genome, has proven to be one of the most powerful experimental tools to understand specific genetic requirements for both developmental and tumor promoting regulatory pathways. These techniques have been enormously useful in elucidating mechanisms of gene regulation and protein function and for establishing direct cell lineage relationships regarding the 'cell of origin' for a number of cancers. Transgenic mice have revealed that overexpression of protooncogenes predispose cells to develop malignant tumors while tumor suppressor genes maintain normal growth control. Transgenic strategies also have revolutionized the way we approach the complex problems associated with tumorigenesis, including issues related to gene regulation, cell-cell interactions, cell cycle control and a variety of signal transduction pathways that impact tumor initiation, progression, and metastatic properties. The Cancer Center Transgenic Mouse Core Facility (TMCF) Shared Resource was established in January 1998 through support from NCI and Purdue University to serve the Purdue Cancer Center research community. The TMCF has grown every year since its inception and is now a state-of-the-art facility that offers the following services; (1) pronuclear injections for the production of transgenic mice, (2) generation of targeted ES cell lines, (3) blastocyst injections to generate chimeric mice for producing homozygous null animal models, (4) strain rederivation, (5) in vitro fertilization, and (6) embryo cryopreservation. The facility also offers chromosome analysis and MEF isolation services. Additionally, a new tetraploid embryo complementation service will be available shortly to generate 100% ES cell-derived mice. The Cancer Center has been an integral component of our cancer research community, fostering close interactions among active scientists in understanding aspects of experimental therapeutics, structural biology, carcinogenesis and gene regulation.

Public Health Relevance

The role of the TMCF is to assist individual investigators and scientific Programs within the Cancer Center that are seeking novel approaches in addressing a variety of cancer related issues. In offering these key transgenic services, the TMCF provides the needed expertise to our investigators to achieve the next challenge in their research goals;goals that are aimed at addressing important cancer biology problems.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-32
Application #
8377157
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
32
Fiscal Year
2012
Total Cost
$137,015
Indirect Cost
$47,169
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Orellana, Esteban A; Tenneti, Srinivasarao; Rangasamy, Loganathan et al. (2017) FolamiRs: Ligand-targeted, vehicle-free delivery of microRNAs for the treatment of cancer. Sci Transl Med 9:
Wyatt-Johnson, Season K; Herr, Seth A; Brewster, Amy L (2017) Status Epilepticus Triggers Time-Dependent Alterations in Microglia Abundance and Morphological Phenotypes in the Hippocampus. Front Neurol 8:700
Han, Ning; Pang, Liang; Xu, Jun et al. (2017) Development of Surface-Variable Polymeric Nanoparticles for Drug Delivery to Tumors. Mol Pharm 14:1538-1547
Wu, M-J; Kim, M R; Chen, Y-S et al. (2017) Retinoic acid directs breast cancer cell state changes through regulation of TET2-PKC? pathway. Oncogene 36:3193-3206
Jang, Yumi; Rao, Xiayu; Jiang, Qing (2017) Gamma-tocotrienol profoundly alters sphingolipids in cancer cells by inhibition of dihydroceramide desaturase and possibly activation of sphingolipid hydrolysis during prolonged treatment. J Nutr Biochem 46:49-56
Li, Jie; Wang, Ruixin; Kong, Yifan et al. (2017) Targeting Plk1 to Enhance Efficacy of Olaparib in Castration-Resistant Prostate Cancer. Mol Cancer Ther 16:469-479
Wang, Siwen; Xing, Zheng; Pascuzzi, Pete E et al. (2017) Metabolic Adaptation to Nutrients Involves Coregulation of Gene Expression by the RNA Helicase Dbp2 and the Cyc8 Corepressor in Saccharomyces cerevisiae. G3 (Bethesda) 7:2235-2247
Yue, Feng; Bi, Pengpeng; Wang, Chao et al. (2017) Pten is necessary for the quiescence and maintenance of adult muscle stem cells. Nat Commun 8:14328
Li, Guo; Low, Philip S (2017) Synthesis and evaluation of a ligand targeting the ? and ? opioid receptors for drug delivery to lung cancer. Bioorg Med Chem Lett 27:2074-2078
Chen, I-Hsuan; Xue, Liang; Hsu, Chuan-Chih et al. (2017) Phosphoproteins in extracellular vesicles as candidate markers for breast cancer. Proc Natl Acad Sci U S A 114:3175-3180

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