The DNA Sequencing Shared Resource serves the Purdue University Center for Cancer Research investigators by providing accurate and timely sequencing of DNA samples. The service is needed for construct verification and sequence analysis by members of all four scientific programs in the Cancer Center and in particular DNA sequencing services critically support the Transgenic Mouse Shared Resource. Instrumentation includes a Nanodrop spectrophotometer and two Applied Biosystems 3730XL genetic analyzers. Results are delivered through web-based interfaces and archived in the shared resource databases. The DNA Sequencing Shared Resource creates and maintains a web page for each laboratory as well as a simple sorting and filtering tool for searching that laboratory's sequences. The tool allows forward and reverse sorting by sample name, primer, date, vector, number of high quality bases and accession number. The DNA Sequencing Shared Resource enjoys wide-spread usage throughout the Cancer and is a cost-effective and convenient essential service.

Public Health Relevance

The role of the shared resource is to assist individual investigators and scientific Programs within the Center that are seeking novel approaches to addressing a variety of cancer-related issues. In offering these key services, the shared resource provides the expertise necessary for achieving the next challenge;challenges that when solved will aid in reducing the pain and suffering of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-33
Application #
8470572
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
33
Fiscal Year
2013
Total Cost
$33,390
Indirect Cost
$11,523
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Hess, David A; Strelau, Katherine M; Karki, Anju et al. (2016) MIST1 Links Secretion and Stress as Both Target and Regulator of the UPR. Mol Cell Biol :
Kim, Myunghoo; Qie, Yaqing; Park, Jeongho et al. (2016) Gut Microbial Metabolites Fuel Host Antibody Responses. Cell Host Microbe 20:202-14
Shan, Tizhong; Zhang, Pengpeng; Xiong, Yan et al. (2016) Lkb1 deletion upregulates Pax7 expression through activating Notch signaling pathway in myoblasts. Int J Biochem Cell Biol 76:31-8
Zhang, Hao; Xing, Zheng; Mani, Saravana Kumar Kailasam et al. (2016) RNA helicase DEAD box protein 5 regulates Polycomb repressive complex 2/Hox transcript antisense intergenic RNA function in hepatitis B virus infection and hepatocarcinogenesis. Hepatology 64:1033-48
Wang, Yang; Zhao, Jing Crystal (2016) Update: Mechanisms Underlying N(6)-Methyladenosine Modification of Eukaryotic mRNA. Trends Genet 32:763-773
Cui, Yi; Wang, Xiaolei; Ren, Wen et al. (2016) Optical Clearing Delivers Ultrasensitive Hyperspectral Dark-Field Imaging for Single-Cell Evaluation. ACS Nano 10:3132-43
Li, J; Gu, D; Lee, S S-Y et al. (2016) Abrogating cholesterol esterification suppresses growth and metastasis of pancreatic cancer. Oncogene 35:6378-6388
Lee, Jaewon; Rancilio, Nicholas J; Poulson, Jean M et al. (2016) Block Copolymer-Encapsulated CaWO4 Nanoparticles: Synthesis, Formulation, and Characterization. ACS Appl Mater Interfaces 8:8608-19
Mishra, A; Maltais, T R; Walter, T M et al. (2016) Trapping and viability of swimming bacteria in an optoelectric trap. Lab Chip 16:1039-46
Rietz, Anne; Petrov, Dino P; Bartolowits, Matthew et al. (2016) Molecular Probing of the HPV-16 E6 Protein Alpha Helix Binding Groove with Small Molecule Inhibitors. PLoS One 11:e0149845

Showing the most recent 10 out of 266 publications