The DNA Sequencing Shared Resource serves the Purdue University Center for Cancer Research investigators by providing accurate and timely sequencing of DNA samples. The service is needed for construct verification and sequence analysis by members of all four scientific programs in the Cancer Center and in particular DNA sequencing services critically support the Transgenic Mouse Shared Resource. Instrumentation includes a Nanodrop spectrophotometer and two Applied Biosystems 3730XL genetic analyzers. Results are delivered through web-based interfaces and archived in the shared resource databases. The DNA Sequencing Shared Resource creates and maintains a web page for each laboratory as well as a simple sorting and filtering tool for searching that laboratory's sequences. The tool allows forward and reverse sorting by sample name, primer, date, vector, number of high quality bases and accession number. The DNA Sequencing Shared Resource enjoys wide-spread usage throughout the Cancer and is a cost-effective and convenient essential service.

Public Health Relevance

The role of the shared resource is to assist individual investigators and scientific Programs within the Center that are seeking novel approaches to addressing a variety of cancer-related issues. In offering these key services, the shared resource provides the expertise necessary for achieving the next challenge;challenges that when solved will aid in reducing the pain and suffering of cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-34
Application #
8681162
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
34
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Wyatt-Johnson, Season K; Herr, Seth A; Brewster, Amy L (2017) Status Epilepticus Triggers Time-Dependent Alterations in Microglia Abundance and Morphological Phenotypes in the Hippocampus. Front Neurol 8:700
Orellana, Esteban A; Tenneti, Srinivasarao; Rangasamy, Loganathan et al. (2017) FolamiRs: Ligand-targeted, vehicle-free delivery of microRNAs for the treatment of cancer. Sci Transl Med 9:
Wu, M-J; Kim, M R; Chen, Y-S et al. (2017) Retinoic acid directs breast cancer cell state changes through regulation of TET2-PKC? pathway. Oncogene 36:3193-3206
Han, Ning; Pang, Liang; Xu, Jun et al. (2017) Development of Surface-Variable Polymeric Nanoparticles for Drug Delivery to Tumors. Mol Pharm 14:1538-1547
Li, Jie; Wang, Ruixin; Kong, Yifan et al. (2017) Targeting Plk1 to Enhance Efficacy of Olaparib in Castration-Resistant Prostate Cancer. Mol Cancer Ther 16:469-479
Jang, Yumi; Rao, Xiayu; Jiang, Qing (2017) Gamma-tocotrienol profoundly alters sphingolipids in cancer cells by inhibition of dihydroceramide desaturase and possibly activation of sphingolipid hydrolysis during prolonged treatment. J Nutr Biochem 46:49-56
Wang, Siwen; Xing, Zheng; Pascuzzi, Pete E et al. (2017) Metabolic Adaptation to Nutrients Involves Coregulation of Gene Expression by the RNA Helicase Dbp2 and the Cyc8 Corepressor in Saccharomyces cerevisiae. G3 (Bethesda) 7:2235-2247
Yue, Feng; Bi, Pengpeng; Wang, Chao et al. (2017) Pten is necessary for the quiescence and maintenance of adult muscle stem cells. Nat Commun 8:14328
Li, Guo; Low, Philip S (2017) Synthesis and evaluation of a ligand targeting the ? and ? opioid receptors for drug delivery to lung cancer. Bioorg Med Chem Lett 27:2074-2078
Chen, I-Hsuan; Xue, Liang; Hsu, Chuan-Chih et al. (2017) Phosphoproteins in extracellular vesicles as candidate markers for breast cancer. Proc Natl Acad Sci U S A 114:3175-3180

Showing the most recent 10 out of 370 publications