The ability to introduce foreign genes into the mammalian germ line, or to selectively ablate endogenous genes from the mouse genome, has proven to be one of the most powerful experimental tools to understand specific genetic requirements for both developmental and tumor promoting regulatory pathways. These techniques have been enormously useful in elucidating mechanisms of gene regulation and protein function and for establishing direct cell lineage relationships regarding the """"""""cell of origin"""""""" for a number of cancers. Transgenic mice have revealed that overexpression of protooncogenes predispose cells to develop malignant tumors while tumor suppressor genes maintain normal growth control. Transgenic strategies also have revolutionized the way we approach the complex problems associated with tumorigenesis, including issues related to gene regulation, cell-cell interactions, cell cycle control and a variety of signal transduction pathways that impact tumor initiation, progression, and metastatic properties. The Cancer Center Transgenic Mouse Core Facility (TMCF) Shared Resource was established in January 1998 through support from NCI and Purdue University to serve the Purdue Cancer Center research community. The TMCF has grown every year since its inception and is now a state-of-the-art facility that offers the following services;(1) pronuclear injections for the production of transgenic mice, (2) generation of targeted ES cell lines, (3) blastocyst injections to generate chimeric mice for producing homozygous null animal models, (4) strain rederivation, (5) in vitro fertilization, and (6) embryo cryopreservation. The facility also offers chromosome analysis and MEF isolation services. Additionally, a new tetraploid embryo complementation service will be available shortly to generate 100% ES cell-derived mice. The Cancer Center has been an integral component of our cancer research community, fostering close interactions among active scientists in understanding aspects of experimental therapeutics, structural biology, carcinogenesis and gene regulation.

Public Health Relevance

The role of the TMCF is to assist individual investigators and scientific Programs within the Cancer Center that are seeking novel approaches in addressing a variety of cancer related issues. In offering these key transgenic services, the TMCF provides the needed expertise to our investigators to achieve the next challenge in their research goals;goals that are aimed at addressing important cancer biology problems.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-34
Application #
8681169
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
34
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Hess, David A; Strelau, Katherine M; Karki, Anju et al. (2016) MIST1 Links Secretion and Stress as Both Target and Regulator of the UPR. Mol Cell Biol :
Kim, Myunghoo; Qie, Yaqing; Park, Jeongho et al. (2016) Gut Microbial Metabolites Fuel Host Antibody Responses. Cell Host Microbe 20:202-14
Shan, Tizhong; Zhang, Pengpeng; Xiong, Yan et al. (2016) Lkb1 deletion upregulates Pax7 expression through activating Notch signaling pathway in myoblasts. Int J Biochem Cell Biol 76:31-8
Zhang, Hao; Xing, Zheng; Mani, Saravana Kumar Kailasam et al. (2016) RNA helicase DEAD box protein 5 regulates Polycomb repressive complex 2/Hox transcript antisense intergenic RNA function in hepatitis B virus infection and hepatocarcinogenesis. Hepatology 64:1033-48
Wang, Yang; Zhao, Jing Crystal (2016) Update: Mechanisms Underlying N(6)-Methyladenosine Modification of Eukaryotic mRNA. Trends Genet 32:763-773
Cui, Yi; Wang, Xiaolei; Ren, Wen et al. (2016) Optical Clearing Delivers Ultrasensitive Hyperspectral Dark-Field Imaging for Single-Cell Evaluation. ACS Nano 10:3132-43
Li, J; Gu, D; Lee, S S-Y et al. (2016) Abrogating cholesterol esterification suppresses growth and metastasis of pancreatic cancer. Oncogene 35:6378-6388
Lee, Jaewon; Rancilio, Nicholas J; Poulson, Jean M et al. (2016) Block Copolymer-Encapsulated CaWO4 Nanoparticles: Synthesis, Formulation, and Characterization. ACS Appl Mater Interfaces 8:8608-19
Mishra, A; Maltais, T R; Walter, T M et al. (2016) Trapping and viability of swimming bacteria in an optoelectric trap. Lab Chip 16:1039-46
Rietz, Anne; Petrov, Dino P; Bartolowits, Matthew et al. (2016) Molecular Probing of the HPV-16 E6 Protein Alpha Helix Binding Groove with Small Molecule Inhibitors. PLoS One 11:e0149845

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