The Flow Cytometry Shared Resource offers Cancer Center users multi-parametric evaluation and high speed enrichment of phenotypically distinct cell populations using flow cytometry. For analytical flow cytometry, investigators have a choice of full-service analysis, or receiving training and then independent use of the two analytical flow cytometers (BD FACSort and BD FACSCanto). Investigator training and consulting is also provided on analytical software including CellQuest, FlowJo, and ModFitLT. For high-speed cell sorting, the facility provides experimental planning and full-service cell sorting by an expert operator, utilizing a BD FACSVantageSE DiVa. This instrument was recently equipped with a custom biosafety cabinet to enable sorting of cells infected with viral vectors, better protecting both the operator and the samples. The experienced Facility Director, Mr. Altman, provides investigators with extensive flow cytometry guidance, from experimental set-up, selection of reagents, setting gating or sorting parameters, data interpretation, and development of new methods. In the previous funding period. Flow Cytometry (previously the major part of the """"""""High Throughput Cell Analysis"""""""" Shared Resource) performed key experiments described in at least 83 publications form Cancer Center members. The Resource is widely used, as in the past year, services were provided for 32 Cancer Center members, representing all four Programs. Plans for future development of the Resource include upgrading the current Flow Cytometers, and acquisition of a low-speed microfluidic sorter that can sort much lower numbers of cells and apply substantially lower shear forces than current instruments. This will be particularly useful for small biopsy samples or sorting for rare slow-growing tumor-initiating cells. In general, experiments performed in the Shared Resource are becoming more operator intensive, and the facility has reached the point where a second staff member is needed for sufficient capacity and optimal support for a diverse and growing base of users. Thus, partial salary support is requested for this technician. $109,057 in CCSG support is requested in the first year, representing 27.3% of the total annual operating budget of the Flow Cytometry Shared Resource.

Public Health Relevance

Flow cytometry enables the analysis of the constituents in complex populations of cells, or the sorting and isolation of particular cells that can shed light on the mechanisms of normal and aberrant cell growth. Centralized and shared flow cytometers under expert supervision can efficiently support a wide range of Cancer Center research.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Sanford-Burnham Medical Research Institute
La Jolla
United States
Zip Code
Fujita, Yu; Khateb, Ali; Li, Yan et al. (2018) Regulation of S100A8 Stability by RNF5 in Intestinal Epithelial Cells Determines Intestinal Inflammation and Severity of Colitis. Cell Rep 24:3296-3311.e6
Scully, Kathleen M; Lahmy, Reyhaneh; Signaevskaia, Lia et al. (2018) E47 Governs the MYC-CDKN1B/p27KIP1-RB Network to Growth Arrest PDA Cells Independent of CDKN2A/p16INK4A and Wild-Type p53. Cell Mol Gastroenterol Hepatol 6:181-198
Borlido, Joana; Sakuma, Stephen; Raices, Marcela et al. (2018) Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nat Immunol 19:594-605
Follis, Ariele Viacava; Llambi, Fabien; Kalkavan, Halime et al. (2018) Regulation of apoptosis by an intrinsically disordered region of Bcl-xL. Nat Chem Biol 14:458-465
Pathria, Gaurav; Scott, David A; Feng, Yongmei et al. (2018) Targeting the Warburg effect via LDHA inhibition engages ATF4 signaling for cancer cell survival. EMBO J 37:
Sun, Younguk; Chen, Bo-Rui; Deshpande, Aniruddha (2018) Epigenetic Regulators in the Development, Maintenance, and Therapeutic Targeting of Acute Myeloid Leukemia. Front Oncol 8:41
Ekanayake, Vindana; Nisan, Danielle; Ryzhov, Pavel et al. (2018) Lipoprotein Particle Formation by Proapoptotic tBid. Biophys J 115:533-542
Diez-Cuñado, Marta; Wei, Ke; Bushway, Paul J et al. (2018) miRNAs that Induce Human Cardiomyocyte Proliferation Converge on the Hippo Pathway. Cell Rep 23:2168-2174
Wang, Yang; Li, Yue; Yue, Minghui et al. (2018) N6-methyladenosine RNA modification regulates embryonic neural stem cell self-renewal through histone modifications. Nat Neurosci 21:195-206
Lundquist, Mark R; Goncalves, Marcus D; Loughran, Ryan M et al. (2018) Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating Autophagy. Mol Cell 70:531-544.e9

Showing the most recent 10 out of 599 publications