Molecular-based imaging provides unique opportunities to assess the pharmacokinetics and targeting properties of r potential therapeutic agents, as well as to assess vital cellular processes in vivo. The ability to monitor the molecular processes of cancer through non-invasive imaging may provide critical information regarding the effects of therapy. In the context of pre-clinical research, the use of in vivo imaging permits the acquisition of a complete dynamic biodistribution study of a molecular tracer on an single animal, thereby reducing the number of animals required to reach a statistically adequate result. Often the imaging techniques and targeting agents that are tested in small animal imaging modalities are directly transferable to the clinical setting. The Small Animal Imaging Core (SAIC) is a shared resource dedicated to providing investigators access to state of the art small animal imaging capabilities for use in basic and translational research relevant to the mission of the City of Hope Cancer Center.
Specific aims of the SAIC include: (1) keeping abreast of the latest developments, current capabilities, and limitations of small animal imaging as pertains to cancer research;(2) implementing, developing, calibrating, maintaining, and operating relevant imaging systems within the context of a small animal imaging laboratory;and (3) optimizing the use of small animal imaging in research at City of Hope in collaboration with investigators. Core personnel currently include a Director, an imaging physicist, and a manager, all of whom are highly experienced in the use of imaging for research with animals. Small animal imaging systems in operation include two units for bioluminescence optical imaging (one has been modified for fluorescence imaging [IVIS 100, Caliper Life Sciences]);a gamma camera (y- IMAGER, Biospace, Inc.);a PET scanner (microPET R4, Siemens);and a CT scanner (microCAT II Hi Res, Siemens). The microPET and microCAT are readily used in tandem to generate co-registered functional anatomic PET/CT images. The Animal Resources Center has provided three rooms within the Parvin Biomedical Research Building for use by the SAIC (one room for the microPET, microCAT, and the y- IMAGER, and two rooms for the IVIS optical imaging instruments. A system has been developed for monitoring instrument usage and to bill users for a portion of the costs of the imaging procedures.

Public Health Relevance

The overall goal of the Small Animal Imaging core facility is to monitor molecular processes of cancer and cancer fighting agents via small animal imaging technologies. This goal enhances the Cancer Center's dedication to developing innovative new disease-fighting strategies in the battle against cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA033572-30
Application #
8450540
Study Section
Subcommittee G - Education (NCI)
Project Start
1997-08-01
Project End
2017-11-30
Budget Start
2013-04-25
Budget End
2013-11-30
Support Year
30
Fiscal Year
2013
Total Cost
$49,651
Indirect Cost
$20,097
Name
City of Hope/Beckman Research Institute
Department
Type
DUNS #
027176833
City
Duarte
State
CA
Country
United States
Zip Code
91010
Nakamura, Ryotaro; La Rosa, Corinna; Longmate, Jeffrey et al. (2016) Viraemia, immunogenicity, and survival outcomes of cytomegalovirus chimeric epitope vaccine supplemented with PF03512676 (CMVPepVax) in allogeneic haemopoietic stem-cell transplantation: randomised phase 1b trial. Lancet Haematol 3:e87-98
Kuo, Ching-Ying; Cheng, Chun-Ting; Hou, Peifeng et al. (2016) HIF-1-alpha links mitochondrial perturbation to the dynamic acquisition of breast cancer tumorigenicity. Oncotarget 7:34052-69
Zhou, Ting; Pan, Feiyan; Cao, Yan et al. (2016) R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation. Oncotarget 7:6902-15
Wittenberg, Elaine; Ferrell, Betty; Goldsmith, Joy et al. (2016) Family Caregiver Communication Tool: a new measure for tailoring communication with cancer caregivers. Psychooncology :
Li, Zhongqi; Oganesyan, Diana; Mooney, Rachael et al. (2016) L-MYC Expression Maintains Self-Renewal and Prolongs Multipotency of Primary Human Neural Stem Cells. Stem Cell Reports 7:483-95
Sun, Virginia; Ruel, Nora; Chung, Vincent et al. (2016) Pilot study of an interdisciplinary supportive care planning intervention in pancreatic cancer. Support Care Cancer 24:3417-24
Leung, Amy; Trac, Candi; Du, Juan et al. (2016) Persistent Chromatin Modifications Induced by High Fat Diet. J Biol Chem 291:10446-55
Reid, Michael A; Lowman, Xazmin H; Pan, Min et al. (2016) IKKβ promotes metabolic adaptation to glutamine deprivation via phosphorylation and inhibition of PFKFB3. Genes Dev 30:1837-51
Champer, Jackson; Ito, James I; Clemons, Karl V et al. (2016) Proteomic Analysis of Pathogenic Fungi Reveals Highly Expressed Conserved Cell Wall Proteins. J Fungi (Basel) 2:
Thomas, Carissa M; Saulnier, Delphine M A; Spinler, Jennifer K et al. (2016) FolC2-mediated folate metabolism contributes to suppression of inflammation by probiotic Lactobacillus reuteri. Microbiologyopen 5:802-818

Showing the most recent 10 out of 1181 publications