The Cell Analysis Facility (CAF) is a fee for service flow cytometry shared resource operating to provide research flow cytometry services to investigators. The goal of the CAF is to provide comprehensive data collection, data analysis, data interpretation, cell sorting, and education in flow cytometry to all Interested investigators. In addition, the facility provides support for proper sample preparation, as well as education in theory and practice of flow cytometry. The CAF provides state of the art flow cytometry support in all areas of established protocols and continually develops new applications guided by investigator needs. Routine analyses include immunophenotyping, DNA/Cell Cycle determinations, fluorescent protein expression, multiple bead based assays for secreted proteins and cell sorting in bulk or single cell cloning. Given the complexity of the equipment, the CAF personnel assist investigators in the design and interpretation of multi laser, multi parametric experiments. The Cell Analysis Facility, located in a 1200 square foot laboratory in the Durham Research Center I (DRCI) Building, was established in 1980 with the aid of a NIH Shared Instrument Grant and a matching grant from the University of Nebraska Foundation. Current instrumentation in the CAF consists of two (2) 4 laser, 15 color BD Bioscience FACS Aria cell sorters, a 4 laser, 15 color BD Biosciences LSR II, three (3) 2 laser, 4 color BD Biosciences FACS Calibur flow cytometers and a 2 laser, 4 color FACS Array. The 4 color BD Biosciences FACS Calibur flow cytometers have served as the main facility instrument for analysis. The increased demand for sorting prompted the purchase of two (2) Becton Dickinson FACS Aria Cell Sorters and a Miltenyi AutoMACS magnetic bead cell sorter during the past 5 years. Additionally, the CAF provides wet lab space for Investigator or facility initiated sample preparation, incubator space for sample storage prior to and following sorting application, and microscopes (fluorescent and bright field) for viewing cells prior to and following sample preparation activities. In support of these instruments, the CAF provides three workstations with additional copies of Modfit, Flowjo, Cellquest and Diva for post acquisition data analysis and graphic generation for publications and grant submissions. Charles Kuszynski, Ph.D. has been the facility director for the Cell Analysis Facility for 30 years. He has significant experience in developing protocols and analyzing data, and he was trained by Becton Dickinson as a field engineer to maintain the instrumentation in the laboratory.

National Institute of Health (NIH)
Center Core Grants (P30)
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Subcommittee B - Comprehensiveness (NCI)
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University of Nebraska Medical Center
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Pysz, Marybeth A; Hao, Fang; Hizli, A Asli et al. (2014) Differential regulation of cyclin D1 expression by protein kinase C ? and ? signaling in intestinal epithelial cells. J Biol Chem 289:22268-83
Schopfer, Lawrence M; Masson, Patrick; Lamourette, Patricia et al. (2014) Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome. Anal Biochem 461:17-26
Kurz, Scott G; Dennison, Kirsten L; Samanas, Nyssa Becker et al. (2014) Ept7 influences estrogen action in the pituitary gland and body weight of rats. Mamm Genome 25:244-52
Zhou, Lin; Anwar, Muhammad M; Zahid, Muhammad et al. (2014) Urinary excretion of N-nitroso compounds in rats fed sodium nitrite and/or hot dogs. Chem Res Toxicol 27:1669-74
Kopanic, Jennifer L; Al-mugotir, Mona H; Kieken, Fabien et al. (2014) Characterization of the connexin45 carboxyl-terminal domain structure and interactions with molecular partners. Biophys J 106:2184-95
Baranovskiy, Andrey G; Gu, Jianyou; Babayeva, Nigar D et al. (2014) Crystallization and preliminary X-ray diffraction analysis of human DNA primase. Acta Crystallogr F Struct Biol Commun 70:206-10
Liu, Xiang; Yi, Chunhui; Wen, Yunfei et al. (2014) Interactions between MUC1 and p120 catenin regulate dynamic features of cell adhesion, motility, and metastasis. Cancer Res 74:1609-20
Jami, Mohammad-Saeid; Hou, Jinxuan; Liu, Miao et al. (2014) Functional proteomic analysis reveals the involvement of KIAA1199 in breast cancer growth, motility and invasiveness. BMC Cancer 14:194
Cavalieri, Ercole; Rogan, Eleanor (2014) The molecular etiology and prevention of estrogen-initiated cancers: Ockham's Razor: Pluralitas non est ponenda sine necessitate. Plurality should not be posited without necessity. Mol Aspects Med 36:1-55
Borgstahl, Gloria E O; Brader, Kerry; Mosel, Adam et al. (2014) Interplay of DNA damage and cell cycle signaling at the level of human replication protein A. DNA Repair (Amst) 21:12-23

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