The Confocal Shared Laser Scanning Microscope Facility (CLSMF) has been active on the UNMC campus since 1996 when it was established through institutional funding. The objectives of the CLSMF are as follows: 1. To fill the need for high resolution image analysis of both live and fixed tissue and cultured cells. 2. To provide training in the use of analysis tools for live cell component analysis of FRET, FRAP and FLIP experiments. 3. To provide essential hard and software for analysis of collected data. 4. To provide the expertise needed both in preparing materials for microscopic examination and in carrying out image generation and analysis The CLSMF is located on the first floor of the Durham Research Center (DRC), Room 1056. The facility contains two Zeiss Confocal Laser Scanning Microscopes. One is a fully motorized LSM 510 META equipped with an Argon Laser (458/477/448/514 nm), a DPSS 561 nm and a HeNe 633nm as well as Nomarski optics. This allows superimposition of signals from fluorphores to be overlain on a DlC image of the cells. Zeiss software is available for collection and analysis of FRET, FRAP and FLIP data, reconstruction of 3-D images, Z-sections, time series, etc. Images can be stored directly on CDs, DVDs, etc. or sent directly to Biomedical Communications by network transfer for production of high resolution dye sublimation prints, slides, posters and illustrations. The Zeis Axiovert inverted microscope used with this system has lOx, 20x, 40x oil, 62x oil and 40x water objectives and a Z-axis motor as well as an xy stage motor. All functions are computer automated. In addition, controlled environment chambers (moisture, C02 levels, temperature) are available for studies of live cells. Judith K. Christman, Ph.D. Is the Facility Director. She has extensive experience in developing immunohistochemistry protocols and In analyzing confocal images. These skills enable her to advise users as to how to optimize their use of confocal microscopy for a variety of different cells and tissues.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA036727-28
Application #
8710010
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
28
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Nebraska Medical Center
Department
Type
DUNS #
City
Omaha
State
NE
Country
United States
Zip Code
68198
Xie, Shuwei; Bahl, Kriti; Reinecke, James B et al. (2016) The endocytic recycling compartment maintains cargo segregation acquired upon exit from the sorting endosome. Mol Biol Cell 27:108-26
Hill, Tanner K; Mohs, Aaron M (2016) Image-guided tumor surgery: will there be a role for fluorescent nanoparticles? Wiley Interdiscip Rev Nanomed Nanobiotechnol 8:498-511
Hanson, Ryan L; Brown, Roger B; Steele, Maria M et al. (2016) Identification of FRA-1 as a novel player in pancreatic cancer in cooperation with a MUC1: ERK signaling axis. Oncotarget 7:39996-40011
Spagnol, Gaelle; Kieken, Fabien; Kopanic, Jennifer L et al. (2016) Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus. J Biol Chem 291:7637-50
Pai, Priya; Rachagani, Satyanarayana; Dhawan, Punita et al. (2016) MUC4 is negatively regulated through the Wnt/β-catenin pathway via the Notch effector Hath1 in colorectal cancer. Genes Cancer 7:154-68
Wuebben, Erin L; Wilder, Phillip J; Cox, Jesse L et al. (2016) SOX2 functions as a molecular rheostat to control the growth, tumorigenicity and drug responses of pancreatic ductal adenocarcinoma cells. Oncotarget 7:34890-906
Hill, Tanner K; Davis, Amanda L; Wheeler, Frances B et al. (2016) Development of a Self-Assembled Nanoparticle Formulation of Orlistat, Nano-ORL, with Increased Cytotoxicity against Human Tumor Cell Lines. Mol Pharm 13:720-8
Marr, Alissa S; Zhang, Chi; Ganti, Apar Kishor (2016) Resected small cell lung cancer-time for more? J Thorac Dis 8:E755-7
Kelkar, Sneha S; Hill, Tanner K; Marini, Frank C et al. (2016) Near infrared fluorescent nanoparticles based on hyaluronic acid: Self-assembly, optical properties, and cell interaction. Acta Biomater 36:112-21
Case, Adam J; Tian, Jun; Zimmerman, Matthew C (2016) Increased mitochondrial superoxide in the brain, but not periphery, sensitizes mice to angiotensin II-mediated hypertension. Redox Biol 11:82-90

Showing the most recent 10 out of 1236 publications