The Gene Targeting Shared Resource was established 10 years ago to assist Cancer Center members who use animal models in constructing and managing genetically altered mouse strains. Located in the Woodbury Animal Shared Resource, this Shared Resource provides services including the generation of transgenic mice by pronuclear injection of DNA, gene targeting in mouse embryonic stem (ES) cells, generation of ES cell chimeras with genetically altered ES cells, and cryopreservation services for both embryos and sperm. To expand on these services further, the facility has recently developed protocols for producing ES cell-derived mice by tetraploid blastocyst injections, a procedure that has greatly facilitated the rapid generation of cancer mouse models at CSHL. It also generated ES cell lines for gene targeting in C57BL/6J mice, which, when fully incorporated, will enable researchers to produce pure mouse strains rapidly in one of the most widely used genetic backgrounds. Finally the Gene Targeting Shared Resource has added karyotyping as a step in the production of chimeric mice;by identifying ES cells with a normal chromosome complement, the efficiency of germline transmission has been greatly enhanced. The rapid incorporation of new technologies within the Resource helps keep research in the Cancer Center at the cutting edge. In addition, its internal development efforts continue to provide Cancer Center investigators with new tools and method to speed up the production of mouse models while maintaining low costs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA045508-24
Application #
8340290
Study Section
Subcommittee G - Education (NCI)
Project Start
2011-08-17
Project End
2016-07-31
Budget Start
2011-08-17
Budget End
2012-07-31
Support Year
24
Fiscal Year
2011
Total Cost
$219,367
Indirect Cost
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
Herrera, Victoria L M; Steffen, Martin; Moran, Ann Marie et al. (2016) Confirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein. BMC Mol Biol 17:15
Fagegaltier, Delphine; Falciatori, Ilaria; Czech, Benjamin et al. (2016) Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway. Genes Dev 30:1623-35
Hossain, Manzar; Stillman, Bruce (2016) Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription. Elife 5:
Ho, Joanne M; Reynolds, Noah M; Rivera, Keith et al. (2016) Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli. ACS Synth Biol 5:163-71
Sheu, Yi-Jun; Kinney, Justin B; Stillman, Bruce (2016) Concerted activities of Mcm4, Sld3, and Dbf4 in control of origin activation and DNA replication fork progression. Genome Res 26:315-30
Arun, Gayatri; Diermeier, Sarah; Akerman, Martin et al. (2016) Differentiation of mammary tumors and reduction in metastasis upon Malat1 lncRNA loss. Genes Dev 30:34-51
Hwang, Chang-Il; Boj, Sylvia F; Clevers, Hans et al. (2016) Preclinical models of pancreatic ductal adenocarcinoma. J Pathol 238:197-204
Baker, Lindsey A; Tiriac, Hervé; Clevers, Hans et al. (2016) Modeling pancreatic cancer with organoids. Trends Cancer 2:176-190
Diermeier, Sarah D; Chang, Kung-Chi; Freier, Susan M et al. (2016) Mammary Tumor-Associated RNAs Impact Tumor Cell Proliferation, Invasion, and Migration. Cell Rep 17:261-74
Nomakuchi, Tomoki T; Rigo, Frank; Aznarez, Isabel et al. (2016) Antisense oligonucleotide-directed inhibition of nonsense-mediated mRNA decay. Nat Biotechnol 34:164-6

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