The Microarray Shared Resource allows the CSHL Cancer Center members to take advantage of powerful genomics tools and techniques. The Shared Resource provides full service microarray analysis, primarily focusing on the Affymetrix and Agilent platforms. The microarray applications used by the facility provide RNA profiling, genome analysis of copy number variation and insights into DNA/protein interactions and chromatin modifications by means of chromatin immunoprecipitation. The use of Microarray services has promoted and strengthened multidisciplinary interactions with the Bioinformatics and DNA Sequencing Shared Resources. Microarrays provide a low resolution, genome-wide view of changes in gene expression in different cell types or under different conditions, which serve as a starting point for pathway analysis and the identification of key regions of variation in cancer gene discovery experiments. In many cases, microarray experiments can be a prelude to high throughput sequencing and can help focus the sequencing efforts.

Public Health Relevance

The Microarray Shared Resource provides an essential and cost effective complement to the other methodologies used at CSHL to characterize genomes and transcriptomes, most notably high-throughput sequencing.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA045508-26
Application #
8473677
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
26
Fiscal Year
2013
Total Cost
$188,548
Indirect Cost
$127,992
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
Herrera, Victoria L M; Steffen, Martin; Moran, Ann Marie et al. (2016) Confirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein. BMC Mol Biol 17:15
Fagegaltier, Delphine; Falciatori, Ilaria; Czech, Benjamin et al. (2016) Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway. Genes Dev 30:1623-35
Hossain, Manzar; Stillman, Bruce (2016) Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription. Elife 5:
Ho, Joanne M; Reynolds, Noah M; Rivera, Keith et al. (2016) Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia coli. ACS Synth Biol 5:163-71
Sheu, Yi-Jun; Kinney, Justin B; Stillman, Bruce (2016) Concerted activities of Mcm4, Sld3, and Dbf4 in control of origin activation and DNA replication fork progression. Genome Res 26:315-30
Arun, Gayatri; Diermeier, Sarah; Akerman, Martin et al. (2016) Differentiation of mammary tumors and reduction in metastasis upon Malat1 lncRNA loss. Genes Dev 30:34-51
Hwang, Chang-Il; Boj, Sylvia F; Clevers, Hans et al. (2016) Preclinical models of pancreatic ductal adenocarcinoma. J Pathol 238:197-204
Baker, Lindsey A; Tiriac, Hervé; Clevers, Hans et al. (2016) Modeling pancreatic cancer with organoids. Trends Cancer 2:176-190
Diermeier, Sarah D; Chang, Kung-Chi; Freier, Susan M et al. (2016) Mammary Tumor-Associated RNAs Impact Tumor Cell Proliferation, Invasion, and Migration. Cell Rep 17:261-74
Nomakuchi, Tomoki T; Rigo, Frank; Aznarez, Isabel et al. (2016) Antisense oligonucleotide-directed inhibition of nonsense-mediated mRNA decay. Nat Biotechnol 34:164-6

Showing the most recent 10 out of 237 publications