Objective: The Flow Cytometry Shared Resource (FCSR) supports translational and clinical cancer research projects of University of Colorado Cancer Center (UCCC) members with flow cytometric analysis, high-speed cell sorting, microscopy, and multiplexed fluorescent microsphere assays. Services and Technologies: FCSR provides a wide range of assays particulariy pertinent to cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis, and cell phenotyping along with cell sorting. Housing 4 high-speed sorters, 8 color analyzers, 1 Luminex system, 1 cell counter, 1 imaging cytometer, 6 microscopes, and 1 confocal scanning system, FCSR has 5,000 and 13,500 hours/year of cell sorting and analysis capacity, respectively;1,500 hours/year of Luminex capacity;and 12,000 hours/year of microscope-based research capacity. Consultation and Training: FCSR provides a consultation to help those members with little flow cytometry or immunology background to design appropriate experiments. In addition, regular training sessions are held to inform UCCC members and the investigators in their laboratories of new techniques and FCSR capabilities. Many investigators also call FCSR on an ad hoc basis for advice on performing experiments. Utilization: FCSR is among the most popular and most highly used of the UCCC shared resources. More than 100 different Cancer Center members from 6 programs and 7 consortium institutions use either the UCD or the NJH branch facilities. Management and Finances: This resource is UCCC-managed. Currently, 7 1% of the operating budget comes from charge backs to UCCC members who represent 76% of Shared Resource users. FCSR requests $220,370 CCSG support for 2 1% of its operating budget Increased funding will expand assay capabilities and will maintain the FCSR's place at the forefront of cytometry technology as the first Beckman Coulter Cytometry Center of Excellence.
Using flow cytometric analysis, high-speed cell sorting, microscopy, and multiplexed fluorescent microsphere assays, the Flow Cytometry Shared Resource can perform a wide range of assays supporting cancer research, including cell cycle, cell proliferation, apoptosis, cell viability, cell signaling, stem cell detection, fluorescent protein analysis, and cell phenotyping. These assays are crucial to understanding how cancer derails normal cellular processes to cause disease.
|Finlay-Schultz, J; Cittelly, D M; Hendricks, P et al. (2015) Progesterone downregulation of miR-141 contributes to expansion of stem-like breast cancer cells through maintenance of progesterone receptor and Stat5a. Oncogene 34:3676-87|
|Alvarez-Calderon, Francesca; Gregory, Mark A; Pham-Danis, Catherine et al. (2015) Tyrosine kinase inhibition in leukemia induces an altered metabolic state sensitive to mitochondrial perturbations. Clin Cancer Res 21:1360-72|
|Kumar, Sushil; Raina, Komal; Agarwal, Chapla et al. (2014) Silibinin strongly inhibits the growth kinetics of colon cancer stem cell-enriched spheroids by modulating interleukin 4/6-mediated survival signals. Oncotarget 5:4972-89|
|Roth, Lauren W; Allshouse, Amanda A; Bradshaw-Pierce, Erica L et al. (2014) Luteal phase dynamics of follicle-stimulating and luteinizing hormones in obese and normal weight women. Clin Endocrinol (Oxf) 81:418-25|
|Roth, Lauren W; Bradshaw-Pierce, Erica L; Allshouse, Amanda A et al. (2014) Evidence of GnRH antagonist escape in obese women. J Clin Endocrinol Metab 99:E871-5|
|Colussi, Timothy M; Costantino, David A; Hammond, John A et al. (2014) The structural basis of transfer RNA mimicry and conformational plasticity by a viral RNA. Nature 511:366-9|
|Griesinger, Andrea M; Donson, Andrew M; Foreman, Nicholas K (2014) Immunotherapeutic implications of the immunophenotype of pediatric brain tumors. Oncoimmunology 3:e27256|
|Marek, Lindsay A; Hinz, Trista K; von Mässenhausen, Anne et al. (2014) Nonamplified FGFR1 is a growth driver in malignant pleural mesothelioma. Mol Cancer Res 12:1460-9|
|Holliday, Michael J; Zhang, Fengli; Isern, Nancy G et al. (2014) 1H, 13C, and 15N backbone and side chain resonance assignments of thermophilic Geobacillus kaustophilus cyclophilin-A. Biomol NMR Assign 8:23-7|
|Milgroom, Andrew; Intrator, Miranda; Madhavan, Krishna et al. (2014) Mesoporous silica nanoparticles as a breast-cancer targeting ultrasound contrast agent. Colloids Surf B Biointerfaces 116:652-7|
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