The Vector Core Facility (VCF) has served, and will continue to serve, as an important shared facility for UPCI researchers by providing many hundreds of viral and non-viral vectors and reagents to UPCI members, particularly for use in cancer gene therapy. Although greater than 50% of the usage of this shared facility is by UPCI members, requested CCSG support is only approximately 15% of the total budget of the facility. The VCF functions within the framework of the UPCI as a dynamic resource that develops novel vectors and provides state-of-the-art viral and non-viral vector technology. The major emphasis of the VCF has been on the utilization of adenoviral and retroviral vectors for gene transduction. This facility also has the capability to produce adeno-associated viruses and DNA plasmids for gene delivery. In addition to vectors, the facility also provides cell lines, viruses, packaging lines, plasmids, and protocols, as well as technical assistance and training to individuals in the use of viral and non-viral vectors for gene transfer. In response to a growth in research demand, over the last year the VCF has expanded its focus to include lentiviral vectors for expression of shRNA and cDNA to aid in studies of gene-discovery, drug development and target validation, and to provide this expertise and reagents to UPCI members involved in basic science research using lentiviral vectors. Currently the VCF provides include high- and low-titer HIV and FIV lentivirus, preparation of stock viruses for fluorescent protein expression and shRNA expression from a library specific to human and mouse genes, design and development of lentiviruses for cDNA expression for cell-based studies and transgenic mouse production, cell transduction & characterization (development of stable cell lines and analysis by qRT-PCR) and lentiviral titering and testing for replication competence.
The specific aims of the Vector Core Facility are to: 1. Provide UPCI investigators with viral and non-viral vectors that express shRNA or the specific gene of interest and that are most appropriate and efficacious for their proposed experiments. 2. Develop improved viral and non-viral vectors for more efficient gene transfer with higher and/or regulated gene expression. 3. Assist in the development of new, state-of-the-art methods for efficient gene delivery. 4. Provide technical assistance and protocols for gene therapy projects, making use of viral and non-viral gene delivery systems.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA047904-25
Application #
8519348
Study Section
Special Emphasis Panel (ZCA1-RTRB-L)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
25
Fiscal Year
2013
Total Cost
$116,335
Indirect Cost
$69,738
Name
University of Pittsburgh
Department
Type
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Zhang, Xuan; Bresee, Jamee; Fields, Gregg B et al. (2014) Near-infrared triple-helical peptide with quenched fluorophores for optical imaging of MMP-2 and MMP-9 proteolytic activity in vivo. Bioorg Med Chem Lett 24:3786-90
Oborski, Matthew J; Demirci, Emre; Laymon, Charles M et al. (2014) Assessment of early therapy response with 18F-FLT PET in glioblastoma multiforme. Clin Nucl Med 39:e431-2
Gross, Neil D; Bauman, Julie E; Gooding, William E et al. (2014) Erlotinib, erlotinib-sulindac versus placebo: a randomized, double-blind, placebo-controlled window trial in operable head and neck cancer. Clin Cancer Res 20:3289-98
Beumer, Jan H; Owzar, Kouros; Lewis, Lionel D et al. (2014) Effect of age on the pharmacokinetics of busulfan in patients undergoing hematopoietic cell transplantation; an alliance study (CALGB 10503, 19808, and 100103). Cancer Chemother Pharmacol 74:927-38
Yang, Liangchun; Xie, Min; Yang, Minghua et al. (2014) PKM2 regulates the Warburg effect and promotes HMGB1 release in sepsis. Nat Commun 5:4436
Sun, Xiaofang; Tang, Daolin (2014) HMGB1-dependent and -independent autophagy. Autophagy 10:1873-6
Pollock, Sheri L; Rush, Elizabeth A; Redner, Robert L (2014) NPM-RAR, not the RAR-NPM reciprocal t(5;17)(q35;q21) acute promyelocytic leukemia fusion protein, inhibits myeloid differentiation. Leuk Lymphoma 55:1383-7
Ng, Yuen-Keng; Lee, Jia-Ying; Supko, Kathryn M et al. (2014) Pan-erbB inhibition potentiates BRAF inhibitors for melanoma treatment. Melanoma Res 24:207-18
Popovtzer, Aron; Normolle, Daniel; Worden, Francis P et al. (2014) Phase I trial of radiotherapy concurrent with twice-weekly gemcitabine for head and neck cancer: translation from preclinical investigations aiming to improve the therapeutic ratio. Transl Oncol 7:479-83
Peterson, Lanell M; Kurland, Brenda F; Schubert, Erin K et al. (2014) A phase 2 study of 16*-[18F]-fluoro-17*-estradiol positron emission tomography (FES-PET) as a marker of hormone sensitivity in metastatic breast cancer (MBC). Mol Imaging Biol 16:431-40

Showing the most recent 10 out of 463 publications