The primary purpose of this shared resource is to generate novel polyclonal and monoclonal antibody reagents, to assist investigators in characterization and optimization of these reagents, and to provide other technical assistance for antibody-related research. Availability of high quality antibody reagents is critical for understanding protein function in normal and neoplastic cells and tissues.
We aim to provide antibodies to cancer center members in a timely manner at a reduced cost, relative to commercial custom antibody services. We can also provide an expanded range of services that are not generally available from commercial sources, so that more investigators without expertise or equipment for molecular cell biology or biochemistry can successfully generate and characterize custom antibody reagents. Our most commonly used services include the generation of rabbit polyclonal antibodies, purification and/or testing of polyclonal antibody, generation of mouse polyclonal antibodies, and the generation, purification, characterization and testing of mouse monoclonal antibodies. Other services that we have provided include production and purification of bacterially expressed fusion protein antigens, antibody isotyping, cryopreservation and storage of hybridoma lines, preparation of Fab fragments from existing antibodies, adaptation of hybridoma lines to serum-free culture and recovery of poorly frozen hybridoma cell lines from other sources.

Public Health Relevance

The mission of the Antibody Shared Resource is to generate novel polyclonal and monoclonal antibody reagents, and to provide antibody-related advice and services to aid cancer center investigators in their research. It is our aim to provide these services in a timely and cost-effective manner.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Texas Health Science Center San Antonio
San Antonio
United States
Zip Code
Ghosh, Sagar; Hughes, Daniel; Parma, Dorothy Long et al. (2014) Association of obesity and circulating adipose stromal cells among breast cancer survivors. Mol Biol Rep 41:2907-16
Meng, Jia; Lu, Zhiliang; Liu, Hui et al. (2014) A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods 69:274-81
Gong, Jingjing; Muñoz, Amanda R; Chan, Daniel et al. (2014) STAT3 down regulates LC3 to inhibit autophagy and pancreatic cancer cell growth. Oncotarget 5:2529-41
Fok, Wilson C; Livi, Carolina; Bokov, Alex et al. (2014) Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. Mech Ageing Dev 140:23-9
Morales, Liza D; Casillas Pavón, Edgar A; Shin, Jun Wan et al. (2014) Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. PLoS One 9:e97104
Mousavi, Seyed Mohsen; Sundquist, Jan; Hemminki, Kari (2014) Risk of Kaposi sarcoma among immigrants to Sweden. Acta Derm Venereol 94:476-7
Ankerst, Donna P; Boeck, Andreas; Freedland, Stephen J et al. (2014) Evaluating the Prostate Cancer Prevention Trial High Grade Prostate Cancer Risk Calculator in 10 international biopsy cohorts: results from the Prostate Biopsy Collaborative Group. World J Urol 32:185-91
Biswas, Tanuka; Gu, Xiang; Yang, Junhua et al. (2014) Attenuation of TGF-* signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346:129-38
Ramirez, Amelie G; Munoz, Edgar; Holden, Alan E C et al. (2014) Incidence of hepatocellular carcinoma in Texas Latinos, 1995-2010: an update. PLoS One 9:e99365
Bansal, H; Yihua, Q; Iyer, S P et al. (2014) WTAP is a novel oncogenic protein in acute myeloid leukemia. Leukemia 28:1171-4

Showing the most recent 10 out of 616 publications