The Cancer Center Flow Cytometry Shared Resource has been integrated in the Institutional Flow Cytometry Core Facility and has been in existence for 23 years. It is located in room 5.044V at the University of Texas Health Science Center at San Antonio (UTHSCSA) and occupies 800 sq. ft. It is administered through the Office of the Vice President for Research in coordination with the Executive Director of the Cancer Center. It serves >35 Cancer Center members in several campuses including the UTHSCSA the Greehey Children's Cancer Research Institute (GCCRI) and the Institute for Drug Discovery (IDD). Access to the flow lab is available to all investigators in the Cancer Center with priority given to NIHfunded -investigators and Cancer Center members. The Flow Cytometry Shared Resource is based on 3 advanced Becton Dickinson (BD) Flow Cytometers: 1) FACSAria cell sorter/ analyzer, equipped with 3- lasers allowing simultaneous sorting and analysis by up to 13 colors, and simultaneous sorting of 4 different cell populations at a speed of up to 20,000 events/sec. 2) FACStar Plus cell sorter/analyzer, equipped with two lasers, and upgraded through an NIH SIG grant to a TurboSort with sorting of 5,000 events/sec and 4-color analysis, and 3) FACSCalibur analyzer, equipped with 2-lasers. Shared resource personnel include the Director with over 30 years.of experience in flow cytometry and two experienced operators (20 and 4 years). All personnel are Becton-Dickinson certified operators. Services include multicolor sorting and analyses, phenotyping and cloning of subpopulations of lymphocytes, tumor cells, stem cells and cells expressing fluorescent transgene proteins;cell sorting according to cell cycle position; measurements and kinetics of nitric oxide, oxygen peroxide and free radicals, reduced glutathione, pH, Ca++ fluxes and fluxes of different vial dyes;quantitation of multiple cytokines using fluorescent microspheres (CBA/BD);study of mitochondrial damage;determination of multiple activated caspases; consulting services;and assistance in grant writing and publication services. The shared resource supports 26 NIH-funded, P30 members holding 20 NIH grants of over $6 million/year in direct costs. Thirty-nine peer reviewed papers were published by P30 members in 2007-2008 (over 150 publications in the past 5 years) with pertinent use of flow cytometry, attesting to the productivity of the cancer investigators and the integral role flow cytometry plays in their ongoing research projects.
The mission of the Flow Cytometry Shared Resource is to enhance basic, preclinical and clinical cancer research. It is the goal of the shared resource to assist individual faculty members in their research endeavors, promoting interdisciplinary and inter-institutional research activities, and the education of graduate students, fellows and clinicians in the Cancer Center and its affiliated institutions. The shared resource is committed to providing professional, efficient, and reasonably priced Flow Cytometry Services.
|Meng, Jia; Lu, Zhiliang; Liu, Hui et al. (2014) A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods 69:274-81|
|Ghosh, Sagar; Hughes, Daniel; Parma, Dorothy Long et al. (2014) Association of obesity and circulating adipose stromal cells among breast cancer survivors. Mol Biol Rep 41:2907-16|
|Fok, Wilson C; Livi, Carolina; Bokov, Alex et al. (2014) Short-term rapamycin treatment in mice has few effects on the transcriptome of white adipose tissue compared to dietary restriction. Mech Ageing Dev 140:23-9|
|Gong, Jingjing; Muñoz, Amanda R; Chan, Daniel et al. (2014) STAT3 down regulates LC3 to inhibit autophagy and pancreatic cancer cell growth. Oncotarget 5:2529-41|
|Mousavi, Seyed Mohsen; Sundquist, Jan; Hemminki, Kari (2014) Risk of Kaposi sarcoma among immigrants to Sweden. Acta Derm Venereol 94:476-7|
|Morales, Liza D; Casillas Pavón, Edgar A; Shin, Jun Wan et al. (2014) Protein tyrosine phosphatases PTP-1B, SHP-2, and PTEN facilitate Rb/E2F-associated apoptotic signaling. PLoS One 9:e97104|
|Biswas, Tanuka; Gu, Xiang; Yang, Junhua et al. (2014) Attenuation of TGF-* signaling supports tumor progression of a mesenchymal-like mammary tumor cell line in a syngeneic murine model. Cancer Lett 346:129-38|
|Ankerst, Donna P; Boeck, Andreas; Freedland, Stephen J et al. (2014) Evaluating the Prostate Cancer Prevention Trial High Grade Prostate Cancer Risk Calculator in 10 international biopsy cohorts: results from the Prostate Biopsy Collaborative Group. World J Urol 32:185-91|
|Bansal, H; Yihua, Q; Iyer, S P et al. (2014) WTAP is a novel oncogenic protein in acute myeloid leukemia. Leukemia 28:1171-4|
|Ramirez, Amelie G; Munoz, Edgar; Holden, Alan E C et al. (2014) Incidence of hepatocellular carcinoma in Texas Latinos, 1995-2010: an update. PLoS One 9:e99365|
Showing the most recent 10 out of 616 publications