Among the keys to deciphering normal physiological processes and their derangements in neoplasia are an understanding of the relationship in spatial and temporal terms of different cellular components to each other, and the related ability to correlate structure with function. Providing these capabilities of visualization is the goal of the KCC Bioimaging Facility/Shared Resource by having available powerful, reliable, and readily accessible light microscopic image acquisition and analysis capabilities for KCC investigators. The Facility is Cancer Center-managed and operates with well-trained staff and a faculty supervisor. Dr. James Keen, and provides training in operation of all instruments as well as consultation in operation, methodology, experimental approach, and interpretation. It is open for scheduled use at any time by trained investigators, or the Facility operator can perform imaging and analysis with laboratory personnel. Through the operation of this Shared Resource, individual KCC investigators are assured of state-of-the-art and reliable facilities operated with a high degree of technical expertise, and are relieved of the obligation for substantial outlay for equipment, maintenance and personnel training. The facility has provided service for more than 90 laboratories and several hundred individuals during the past award period, of which more than 80% are Cancer Center members. Major support for the Facility has been derived from successful competition for extramural funding, from institutional and Cancer Center sources, and from user chargebacks tied to services rendered.

Public Health Relevance

The Kimmel Cancer Center Bioimaging Facility provides investigators with the capability to visualize cells and structures within living and fixed cells at the light microscope level. By being able to determine the location and distribution of cellular components in time and space in normal and neoplastic samples, investigators can gain new information about how cancer cells differ from normal ones, and test hypotheses about cancer causation and progression.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA056036-15
Application #
8753663
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
15
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Thomas Jefferson University
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19107
Hussain, Maha; Daignault-Newton, Stephanie; Twardowski, Przemyslaw W et al. (2018) Targeting Androgen Receptor and DNA Repair in Metastatic Castration-Resistant Prostate Cancer: Results From NCI 9012. J Clin Oncol 36:991-999
Shafi, Ayesha A; Schiewer, Matthew J; de Leeuw, Renée et al. (2018) Patient-derived Models Reveal Impact of the Tumor Microenvironment on Therapeutic Response. Eur Urol Oncol 1:325-337
Meyer, Sara E; Muench, David E; Rogers, Andrew M et al. (2018) miR-196b target screen reveals mechanisms maintaining leukemia stemness with therapeutic potential. J Exp Med 215:2115-2136
Mazina, Olga M; Mazin, Alexander V (2018) Reconstituting the 4-Strand DNA Strand Exchange. Methods Enzymol 600:285-305
Magee, Michael S; Abraham, Tara S; Baybutt, Trevor R et al. (2018) Human GUCY2C-Targeted Chimeric Antigen Receptor (CAR)-Expressing T Cells Eliminate Colorectal Cancer Metastases. Cancer Immunol Res 6:509-516
Chervoneva, Inna; Freydin, Boris; Hyslop, Terry et al. (2018) Modeling qRT-PCR dynamics with application to cancer biomarker quantification. Stat Methods Med Res 27:2581-2595
Capparelli, Claudia; Purwin, Timothy J; Heilman, Shea A et al. (2018) ErbB3 Targeting Enhances the Effects of MEK Inhibitor in Wild-Type BRAF/NRAS Melanoma. Cancer Res 78:5680-5693
Nevler, Avinoam; Muller, Alexander J; Cozzitorto, Joseph A et al. (2018) A Sub-Type of Familial Pancreatic Cancer: Evidence and Implications of Loss-of-Function Polymorphisms in Indoleamine-2,3-Dioxygenase-2. J Am Coll Surg 226:596-603
Peng, Weidan; Furuuchi, Narumi; Aslanukova, Ludmila et al. (2018) Elevated HuR in Pancreas Promotes a Pancreatitis-Like Inflammatory Microenvironment That Facilitates Tumor Development. Mol Cell Biol 38:
Waldman, Scott A; Camilleri, Michael (2018) Guanylate cyclase-C as a therapeutic target in gastrointestinal disorders. Gut 67:1543-1552

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