The Robert H. Lurie Comprehensive Cancer Center Mouse Phenotyping Core Facility provides comprehensive gross and histopathologic assessment of murine phenotypes, histological evaluation of tissue derived from animal tumor models, and training sessions for investigators to learn to detect gross anomalies in mice, harvest tissue from various organ systems, and to perform immunohistochemical or special stains on tissue sections. This Core Facility was developed to fulfill a need by the investigative community to accurately analyze their new murine models and to enhance the ability to extract meaningful phenotypic information to guide future investigations. Murine tissue has histological characteristics distinct from human tissue;therefore, assessment under the microscope requires pathologists with experience in murine histology. Moreover, tissue harvesting, processing, and sectioning demands precision, especially in the case of embryonic lethal phenotypes. The Core Facility is directed by a perinatal pathologist with more than fifteen years of experience in animal tissue assessment, immunohistochemical analyses, and pathology. Her extensive background in developmental anomalies allows for accurate assessment of embryonic lethal phenotypes and for establishing the proximate cause of the demise. Other team members of this facility include a second perinatal pathologist, a molecular biologist, and a certified histotechnologist. Together, this team provides invaluable expertise to investigators by offering insight into phenotypes of newly developed murine models, by providing training sessions in tissue harvesting and analysis, and by assisting with performance and grading of immunohistochemical studies. This comprehensive approach to phenotyping viable and embryonic lethal models has the potential to discover new target organ systems and signaling partners for molecules and to identify innovative model systems to study cancer biology for the scientific community.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
3P30CA060553-18S3
Application #
8486523
Study Section
Subcommittee G - Education (NCI)
Project Start
2012-09-06
Project End
2013-07-31
Budget Start
2012-09-06
Budget End
2013-07-31
Support Year
18
Fiscal Year
2012
Total Cost
$161,721
Indirect Cost
$85,187
Name
Northwestern University at Chicago
Department
Type
DUNS #
005436803
City
Chicago
State
IL
Country
United States
Zip Code
60611
Moinpour, Carol M; Donaldson, Gary W; Davis, Kimberly M et al. (2017) The challenge of measuring intra-individual change in fatigue during cancer treatment. Qual Life Res 26:259-271
Lamar, Tyra; Vanoye, Carlos G; Calhoun, Jeffrey et al. (2017) SCN3A deficiency associated with increased seizure susceptibility. Neurobiol Dis 102:38-48
Yu, Dou; Khan, Omar F; SuvĂ , Mario L et al. (2017) Multiplexed RNAi therapy against brain tumor-initiating cells via lipopolymeric nanoparticle infusion delays glioblastoma progression. Proc Natl Acad Sci U S A 114:E6147-E6156
Mohr, David C; Tomasino, Kathryn Noth; Lattie, Emily G et al. (2017) IntelliCare: An Eclectic, Skills-Based App Suite for the Treatment of Depression and Anxiety. J Med Internet Res 19:e10
Apple, Alexandra C; Ryals, Anthony J; Alpert, Kathryn I et al. (2017) Subtle hippocampal deformities in breast cancer survivors with reduced episodic memory and self-reported cognitive concerns. Neuroimage Clin 14:685-691
Lampe, Johanna W; Huang, Ying; Neuhouser, Marian L et al. (2017) Dietary biomarker evaluation in a controlled feeding study in women from the Women's Health Initiative cohort. Am J Clin Nutr 105:466-475
Ichikawa, Yuichi; Connelly, Caitlin F; Appleboim, Alon et al. (2017) A synthetic biology approach to probing nucleosome symmetry. Elife 6:
Zhou, Qiyuan; Dai, Jingbo; Chen, Tianji et al. (2017) Downregulation of PKC?/Pard3/Pard6b is responsible for lung adenocarcinoma cell EMT and invasion. Cell Signal 38:49-59
Park, Jong Kook; Peng, Han; Yang, Wending et al. (2017) miR-184 exhibits angiostatic properties via regulation of Akt and VEGF signaling pathways. FASEB J 31:256-265
Raji, Idris; Yadudu, Fatima; Janeira, Emily et al. (2017) Bifunctional conjugates with potent inhibitory activity towards cyclooxygenase and histone deacetylase. Bioorg Med Chem 25:1202-1218

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