The High Throughput Analysis (HTA) Laboratory was a developmental facility in the past CCSG renewal, in which it was referred to as the "Diagnostics and Therapeutics Screening Core". It is located on the Evanston Campus. The HTA Laboratory's mission is to provide Lurie Cancer Center researchers with advanced technology and expertise for large scale experiments that elucidate the basic biology of cancer and discover novel therapeutic agents. Such experiments can involve parallel manipulation of thousands of samples and the gathering and analysis of data at a similar scale. They include cell-based screening of large compound libraries, massively parallel investigation of gene function using RNAi and gene disruption collections, and high throughput biochemical assays. The facility offers advanced technologies for automated liquid handling, strain collection manipulation, and data acquisition. It provides access to 17 major instruments. These include four advanced platforms for robotic liquid handling, two of which specialize in highly accurate nanoliter liquid dispensing. The facility's analytical equipment includes four photometric plate readers that provide facility users all major detection modes, including simultaneous whole-plate fluorescence measurement for parallel analysis of ion currents and other kinetic phenomena. Additionally, the facility offers access to a Cellomics Arrayscan high-content screening system. The HTA Laboratory integrates these platforms with laboratory infrastructure such as tissue culture, wet bench, and microbial growth chambers, allowing complex in-house work flows. Together these make a broad spectrum of molecular and cell-based assays possible. Importantly, In addition to training and access to instruments, the core offers extensive service for design, validation, and execution of diverse large-scale experiments, including compound library screening and genome-scale functional analysis. The facility also houses biological and chemical libraries for distribution or screening, including a collection of bacterial stocks of snRNAmir lentiviral vector constructs that target nearly all genes in the human and mouse genomes. This capability includes a recently installed tube-based chemical library storage system The HTA Laboratory currently has 251 active users from 76 research groups. Over the last funding period, 79 to 95% of instrument was by Cancer Center member groups. Thus, in its developmental period the facility has become an important established resource for the cancer research community at Northwestern University.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Northwestern University at Chicago
United States
Zip Code
Caralt, M; Uzarski, J S; Iacob, S et al. (2015) Optimization and critical evaluation of decellularization strategies to develop renal extracellular matrix scaffolds as biological templates for organ engineering and transplantation. Am J Transplant 15:64-75
Hung, Andy H; Holbrook, Robert J; Rotz, Matthew W et al. (2014) Graphene oxide enhances cellular delivery of hydrophilic small molecules by co-incubation. ACS Nano 8:10168-77
Gach, Johannes S; Achenbach, Chad J; Chromikova, Veronika et al. (2014) HIV-1 specific antibody titers and neutralization among chronically infected patients on long-term suppressive antiretroviral therapy (ART): a cross-sectional study. PLoS One 9:e85371
Zhao, Baobing; Keerthivasan, Ganesan; Mei, Yang et al. (2014) Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis. Haematologica 99:1157-67
Keller, Jacob Pearson; Homma, Kazuaki; Duan, Chongwen et al. (2014) Functional regulation of the SLC26-family protein prestin by calcium/calmodulin. J Neurosci 34:1325-32
Daugherty, Rebecca L; Serebryannyy, Leonid; Yemelyanov, Alex et al. (2014) ?-Catenin is an inhibitor of transcription. Proc Natl Acad Sci U S A 111:5260-5
Jensen, Samuel A; Calvert, Andrea E; Volpert, Giora et al. (2014) Bcl2L13 is a ceramide synthase inhibitor in glioblastoma. Proc Natl Acad Sci U S A 111:5682-7
Cull, Elizabeth H; Altman, Jessica K (2014) Contemporary treatment of APL. Curr Hematol Malig Rep 9:193-201
Anderson, Mark T; Dewenter, Lena; Maier, Berenike et al. (2014) Seminal plasma initiates a Neisseria gonorrhoeae transmission state. MBio 5:e01004-13
Gopal, Satish; Achenbach, Chad J; Yanik, Elizabeth L et al. (2014) Moving forward in HIV-associated cancer. J Clin Oncol 32:876-80

Showing the most recent 10 out of 316 publications