- Transgenic Mouse Facility (TMF) The Transgenic Mouse Facility (TMF) shared resource communicates awareness about existing and emerging methods and resources involving genetically modified mice to Cancer Center investigators and helps them incorporate these tools into their research programs. The TMF provides services on a recharge basis that require specialized training, are technically difficult to perform, or require expensive equipment. The services include design, development, re-derivation, cryopreservation, and re-animation of genetically modified mice in an efficient and cost-effective manner. The TMF website (http://research.uci.edu/tmf/index.htm) lists information about new resources from the literature as well as available services including (a) consultation on strategies to modify the mouse genome, (b) insertion of conventional multi-copy transgenes and bacterial artificial chromosomes (BAC) at random loci via pronuclear injection of DNA, (c) targeted insertion of single- copy transgenes at the ROSA26 locus via homologous recombination in ES cells, (d) targeted mutagenesis of endogenous loci in ES cells, (e) targeted mutagenesis of loci in mice via Cas-9, (f) cryopreservation of mouse sperm and preimplantation-stage embryos, (g) importation, export, rederivation or reanimation of mouse strains via IVF or embryo transfer, (h) breeding and genotyping of mouse strains, (i) Southern blot analysis of targeted loci in ES cells and animals. The TMF also collaborates with CFCCC members to develop or import new methods involving genetically modified mice at no initial cost to the investigator. Consultation regarding projects continues to be provided at no cost. In addition to CFCCC Members, the TMF supports investigators at many different universities and corporations within the USA. These include academic institutions (UCLA, UCSC, UCSB, UCR, UCSF, Stanford, USC, VA) and corporations (Irvine Scientific, Isis Pharmaceuticals, Peregrine Pharmaceuticals) in CA. The TMF also services investigators at institutions in 17 additional states, including 7 other NCI-designated CCCs: UCLA- Jonsson; UCSF - Diller; OHSU - Knight; MUSC - Hollings; Stanford Cancer Institute; St. Jude's Children's Research Center, USC-Norris. A link on the TMF home page invites investigators to contact the TMF Managing Director and Scientific Director to ask about resources for their use, including advice on mouse models of cancer, identifying and importing strains of mice with cancer susceptibility, and mouse genetics resources of use in studying the cancer problem. TMF personnel subsequently identify resources to meet the researcher's needs and provide these at the lowest possible cost.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA062203-19
Application #
8999872
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
1997-09-11
Project End
2021-01-31
Budget Start
2016-02-01
Budget End
2017-01-31
Support Year
19
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92617
Yu, James; Landberg, Jenny; Shavarebi, Farbod et al. (2018) Bioengineering triacetic acid lactone production in Yarrowia lipolytica for pogostone synthesis. Biotechnol Bioeng 115:2383-2388
Oliver, Andrew; Kay, Matthew; Cooper, Kerry K (2018) Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation. BMC Genomics 19:310
Mahlbacher, Grace; Curtis, Louis T; Lowengrub, John et al. (2018) Mathematical modeling of tumor-associated macrophage interactions with the cancer microenvironment. J Immunother Cancer 6:10
Neek, Medea; Tucker, Jo Anne; Kim, Tae Il et al. (2018) Co-delivery of human cancer-testis antigens with adjuvant in protein nanoparticles induces higher cell-mediated immune responses. Biomaterials 156:194-203
McLelland, Bryce T; Lin, Bin; Mathur, Anuradha et al. (2018) Transplanted hESC-Derived Retina Organoid Sheets Differentiate, Integrate, and Improve Visual Function in Retinal Degenerate Rats. Invest Ophthalmol Vis Sci 59:2586-2603
Bota, Daniela A; Chung, Jinah; Dandekar, Manisha et al. (2018) Phase II study of ERC1671 plus bevacizumab versus bevacizumab plus placebo in recurrent glioblastoma: interim results and correlations with CD4+ T-lymphocyte counts. CNS Oncol 7:CNS22
Han, Han; Qi, Ruxi; Zhou, Jeff Jiajing et al. (2018) Regulation of the Hippo Pathway by Phosphatidic Acid-Mediated Lipid-Protein Interaction. Mol Cell 72:328-340.e8
Solares, Edwin A; Chakraborty, Mahul; Miller, Danny E et al. (2018) Rapid Low-Cost Assembly of the Drosophila melanogaster Reference Genome Using Low-Coverage, Long-Read Sequencing. G3 (Bethesda) 8:3143-3154
Wang, Yajun; Ngor, Arlene K; Nikoomanzar, Ali et al. (2018) Evolution of a General RNA-Cleaving FANA Enzyme. Nat Commun 9:5067
Qiu, Xiaolong; Lombardo, Jeremy A; Westerhof, Trisha M et al. (2018) Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells. Lab Chip 18:2776-2786

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