The Transgenic Mouse Facility (TMF) shared resource communicates awareness about existing and emergingmethods and resources involving genetically modified mice to Cancer Center investigators and helps themincorporate these tools into their research programs. The TMF provides services on a recharge basis thatrequire specialized training, are technically difficult to perform, or require expensive equipment. The servicesinclude design, development, re-derivation, cryopreservation, and re-animation of genetically modified mice inan efficient and cost-effective manner. The TMF website (http://research.uci.edu/tmf/index.htm) listsinformation about new resources from the literature as well as available services including (a) consultation onstrategies to modify the mouse genome, (b) insertion of conventional multi-copy transgenes and bacterialartificial chromosomes (BAC) at random loci via pronuclear injection of DNA, (c) targeted insertion of singlecopytransgenes at the ROSA26 locus via homologous recombination in ES cells, (d) targeted mutagenesis ofendogenous loci in ES cells, (e) targeted mutagenesis of loci in mice via Cas-9, (f) cryopreservation of mousesperm and preimplantation-stage embryos, (g) importation, export, rederivation or reanimation of mousestrains via IVF or embryo transfer, (h) breeding and genotyping of mouse strains, (i) Southern blot analysis oftargeted loci in ES cells and animals. The TMF also collaborates with CFCCC members to develop or importnew methods involving genetically modified mice at no initial cost to the investigator. Consultation regardingprojects continues to be provided at no cost. In addition to CFCCC Members, the TMF supports investigatorsat many different universities and corporations within the USA. These include academic institutions (UCLA,UCSC, UCSB, UCR, UCSF, Stanford, USC, VA) and corporations (Irvine Scientific, Isis Pharmaceuticals,Peregrine Pharmaceuticals) in CA. The TMF also services investigators at institutions in 17 additional states,including 7 other NCI-designated CCCs: UCLA- Jonsson; UCSF - Diller; OHSU - Knight; MUSC - Hollings;Stanford Cancer Institute; St. Jude's Children's Research Center, USC-Norris. A link on the TMF home pageinvites investigators to contact the TMF Managing Director and Scientific Director to ask about resources fortheir use, including advice on mouse models of cancer, identifying and importing strains of mice with cancersusceptibility, and mouse genetics resources of use in studying the cancer problem. TMF personnelsubsequently identify resources to meet the researcher?s needs and provide these at the lowest possible cost.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA062203-20
Application #
9208756
Study Section
Subcommittee A - Cancer Centers (NCI-A)
Project Start
Project End
Budget Start
2017-02-01
Budget End
2018-01-31
Support Year
20
Fiscal Year
2017
Total Cost
$35,870
Indirect Cost
$12,654
Name
University of California Irvine
Department
Type
Domestic Higher Education
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92617
Chakraborty, Mahul; VanKuren, Nicholas W; Zhao, Roy et al. (2018) Hidden genetic variation shapes the structure of functional elements in Drosophila. Nat Genet 50:20-25
Morozko, Eva L; Ochaba, Joseph; Hernandez, Sarah J et al. (2018) Longitudinal Biochemical Assay Analysis of Mutant Huntingtin Exon 1 Protein in R6/2 Mice. J Huntingtons Dis 7:321-335
Carpenter, Philip M; Ziogas, Argyrios; Markham, Emma M et al. (2018) Laminin 332 expression and prognosis in breast cancer. Hum Pathol 82:289-296
Yan, Huaming; Romero-López, Mónica; Benitez, Lesly I et al. (2018) Multiscale modeling of glioblastoma. Transl Cancer Res 7:S96-S98
Yu, James; Landberg, Jenny; Shavarebi, Farbod et al. (2018) Bioengineering triacetic acid lactone production in Yarrowia lipolytica for pogostone synthesis. Biotechnol Bioeng 115:2383-2388
Oliver, Andrew; Kay, Matthew; Cooper, Kerry K (2018) Comparative genomics of cocci-shaped Sporosarcina strains with diverse spatial isolation. BMC Genomics 19:310
Mahlbacher, Grace; Curtis, Louis T; Lowengrub, John et al. (2018) Mathematical modeling of tumor-associated macrophage interactions with the cancer microenvironment. J Immunother Cancer 6:10
Neek, Medea; Tucker, Jo Anne; Kim, Tae Il et al. (2018) Co-delivery of human cancer-testis antigens with adjuvant in protein nanoparticles induces higher cell-mediated immune responses. Biomaterials 156:194-203
McLelland, Bryce T; Lin, Bin; Mathur, Anuradha et al. (2018) Transplanted hESC-Derived Retina Organoid Sheets Differentiate, Integrate, and Improve Visual Function in Retinal Degenerate Rats. Invest Ophthalmol Vis Sci 59:2586-2603
Bota, Daniela A; Chung, Jinah; Dandekar, Manisha et al. (2018) Phase II study of ERC1671 plus bevacizumab versus bevacizumab plus placebo in recurrent glioblastoma: interim results and correlations with CD4+ T-lymphocyte counts. CNS Oncol 7:CNS22

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