- Transgenic Mouse Facility (TMF) The Transgenic Mouse Facility (TMF) shared resource communicates awareness about existing and emerging methods and resources involving genetically modified mice to Cancer Center investigators and helps them incorporate these tools into their research programs. The TMF provides services on a recharge basis that require specialized training, are technically difficult to perform, or require expensive equipment. The services include design, development, re-derivation, cryopreservation, and re-animation of genetically modified mice in an efficient and cost-effective manner. The TMF website (http://research.uci.edu/tmf/index.htm) lists information about new resources from the literature as well as available services including (a) consultation on strategies to modify the mouse genome, (b) insertion of conventional multi-copy transgenes and bacterial artificial chromosomes (BAC) at random loci via pronuclear injection of DNA, (c) targeted insertion of single- copy transgenes at the ROSA26 locus via homologous recombination in ES cells, (d) targeted mutagenesis of endogenous loci in ES cells, (e) targeted mutagenesis of loci in mice via Cas-9, (f) cryopreservation of mouse sperm and preimplantation-stage embryos, (g) importation, export, rederivation or reanimation of mouse strains via IVF or embryo transfer, (h) breeding and genotyping of mouse strains, (i) Southern blot analysis of targeted loci in ES cells and animals. The TMF also collaborates with CFCCC members to develop or import new methods involving genetically modified mice at no initial cost to the investigator. Consultation regarding projects continues to be provided at no cost. In addition to CFCCC Members, the TMF supports investigators at many different universities and corporations within the USA. These include academic institutions (UCLA, UCSC, UCSB, UCR, UCSF, Stanford, USC, VA) and corporations (Irvine Scientific, Isis Pharmaceuticals, Peregrine Pharmaceuticals) in CA. The TMF also services investigators at institutions in 17 additional states, including 7 other NCI-designated CCCs: UCLA- Jonsson; UCSF - Diller; OHSU - Knight; MUSC - Hollings; Stanford Cancer Institute; St. Jude's Children's Research Center, USC-Norris. A link on the TMF home page invites investigators to contact the TMF Managing Director and Scientific Director to ask about resources for their use, including advice on mouse models of cancer, identifying and importing strains of mice with cancer susceptibility, and mouse genetics resources of use in studying the cancer problem. TMF personnel subsequently identify resources to meet the researcher's needs and provide these at the lowest possible cost.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Irvine
United States
Zip Code
Charney, Rebekah M; Forouzmand, Elmira; Cho, Jin Sun et al. (2017) Foxh1 Occupies cis-Regulatory Modules Prior to Dynamic Transcription Factor Interactions Controlling the Mesendoderm Gene Program. Dev Cell 40:595-607.e4
HD iPSC Consortium (2017) Developmental alterations in Huntington's disease neural cells and pharmacological rescue in cells and mice. Nat Neurosci 20:648-660
McCracken, A N; McMonigle, R J; Tessier, J et al. (2017) Phosphorylation of a constrained azacyclic FTY720 analog enhances anti-leukemic activity without inducing S1P receptor activation. Leukemia 31:669-677
Barton, James C; Chen, Wen-Pin; Emond, Mary J et al. (2017) GNPAT p.D519G is independently associated with markedly increased iron stores in HFE p.C282Y homozygotes. Blood Cells Mol Dis 63:15-20
Yan, Huaming; Romero-Lopez, Monica; Frieboes, Hermann B et al. (2017) Multiscale Modeling of Glioblastoma Suggests that the Partial Disruption of Vessel/Cancer Stem Cell Crosstalk Can Promote Tumor Regression Without Increasing Invasiveness. IEEE Trans Biomed Eng 64:538-548
Klempner, Samuel J; Mehta, Pareen; Schrock, Alexa B et al. (2017) Cis-oriented solvent-front EGFR G796S mutation in tissue and ctDNA in a patient progressing on osimertinib: a case report and review of the literature. Lung Cancer (Auckl) 8:241-247
Molino, Nicholas M; Neek, Medea; Tucker, Jo Anne et al. (2017) Display of DNA on Nanoparticles for Targeting Antigen Presenting Cells. ACS Biomater Sci Eng 3:496-501
Lomeli, Naomi; Di, Kaijun; Czerniawski, Jennifer et al. (2017) Cisplatin-induced mitochondrial dysfunction is associated with impaired cognitive function in rats. Free Radic Biol Med 102:274-286
Huang, Jason Y; Samarasena, Jason B; Tsujino, Takeshi et al. (2017) EUS-guided portal pressure gradient measurement with a simple novel device: a human pilot study. Gastrointest Endosc 85:996-1001
Elbir, Haitham; Sitlani, Parth; Bergström, Sven et al. (2017) Chromosome and Megaplasmid Sequences of Borrelia anserina (Sakharoff 1891), the Agent of Avian Spirochetosis and Type Species of the Genus. Genome Announc 5:

Showing the most recent 10 out of 1031 publications