The Flow Cytometry Shared Resource (FCSR) offers a variety of cell-based research services, principally analytical and sorting flow cytometry. The instrumentation necessary for high-speed cell analysis and sorting is expensive and requires a high level of technical expertise; therefore, this equipment is best situated in a shared resource. FCSR provides for the maintenance of the instrumentation, operafion of the resource, and training and consultation in the use of fiow cytometry in experimental work. The staff offers guidance and training to faculty, staff, professional trainees and students in the entire range of skills needed to utilize flow cytometry, including experiment design, sample preparation and staining, data acquisition, post-acquisition analysis, and sorting. The staff organizes regular training sessions for investigators, given by visiting technical specialists or by the staff themselves. FCSR provides materials related to investigators' data and approaches that can support the feasibility of experiments for grant applications, and provides publication quality representations of primary data when needed. Because the personnel and directors of the FCSR have been active in development of new techniques in fiow cytometry, they have relationships with corporations for the development of new techniques in fluorescence detecfion and instrumentation and with other investigators for novel bioinformatics approaches. This unique feature facilitates rapid adoption of new protocols and techniques. Dr. James E. Crowe Jr. is FCSR scientific director. He meets regularly with staff to review FCSR performance, policies, billing and budget status, personnel issues, protocols and procedures, quality assurance and quality control, and developmental studies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA068485-16
Application #
8379920
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-09-01
Budget End
2013-08-31
Support Year
16
Fiscal Year
2012
Total Cost
$83,617
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Paul, Pritha; Rellinger, Eric J; Qiao, Jingbo et al. (2017) Elevated TIMP-1 expression is associated with a prometastatic phenotype, disease relapse, and poor survival in neuroblastoma. Oncotarget 8:82609-82620
Stephenson, Jason R; Wang, Xiaohan; Perfitt, Tyler L et al. (2017) A Novel Human CAMK2A Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. J Neurosci 37:2216-2233
Bouziat, Romain; Hinterleitner, Reinhard; Brown, Judy J et al. (2017) Reovirus infection triggers inflammatory responses to dietary antigens and development of celiac disease. Science 356:44-50
Moyo, T K; Wilson, C S; Moore, D J et al. (2017) Myc enhances B-cell receptor signaling in precancerous B cells and confers resistance to Btk inhibition. Oncogene 36:4653-4661
Vilgelm, Anna E; Cobb, Priscilla; Malikayil, Kiran et al. (2017) MDM2 Antagonists Counteract Drug-Induced DNA Damage. EBioMedicine 24:43-55
LePage, Daniel P; Metcalf, Jason A; Bordenstein, Sarah R et al. (2017) Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility. Nature 543:243-247
Li, Jun; Smith, Jarrod A; Dawson, Eric S et al. (2017) Optimized Translocator Protein Ligand for Optical Molecular Imaging and Screening. Bioconjug Chem 28:1016-1023
Zhang, Juliet; Weinrich, Jarret A P; Russ, Jeffrey B et al. (2017) A Role for Dystonia-Associated Genes in Spinal GABAergic Interneuron Circuitry. Cell Rep 21:666-678
Aune, T M; Crooke 3rd, P S; Patrick, A E et al. (2017) Expression of long non-coding RNAs in autoimmunity and linkage to enhancer function and autoimmune disease risk genetic variants. J Autoimmun 81:99-109
Esbenshade, Adam J; Zhao, Zhiguo; Aftandilian, Catherine et al. (2017) Multisite external validation of a risk prediction model for the diagnosis of blood stream infections in febrile pediatric oncology patients without severe neutropenia. Cancer 123:3781-3790

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