The mission of the Biomolecular &Proteomics Shared Resource (BPSR) is to provide cost-effective, state-of-the-art instrumentation and analytical expertise to investigators in the Vanderbilt-lngram Cancer Center (VICC). The shared resource consists of three specialized laboratories dedicated to analysis of small molecules (i.e., drugs, drug metabolites and endogenous metabolites) In tissues and physiologic fluids;a comprehensive analytical proteomics facility for identification, characterization and quantitation of proteins; and a mass spectrometry imaging and histology-directed protein profiling facility. At the time of the last competitive renewal, these activities were separated into three independent shared resources, each receiving an """"""""Outstanding"""""""" rating. Combining them into a single administrative structure has improved efficiency and service, while cutting costs. The facility currently houses 17 mass spectrometers. Other specialized capital equipment for imaging and proteomic studies are also available, such as high resolution matrix spotters, a cryomacrotome for whole animal imaging studies, a high resolution digital microscope for documenting histology data, a 2D gel fluorescence imaging and 2D gel processing robot. A bioinformatics support staff develops new software tools to support the shared resource and maintains the computing infrastructure. Justifications for including this shared resource in the CCSG proposal include (1) the extensive use of mass spectrometry services by the VICC research community, (2) the unique and comprehensive array of services offered to investigators, and (3) the cost-effective delivery of these services by a highly skilled professional staff. In FY 2008, 95 VICC/CCSG research groups used these services. The shared resource provides 16 categories of analytical services covering a wide range of analytical measurements from drug quantitation for PK/PD studies;identification, characterization and quantitation of cancer biomarkers;and imaging and profiling of proteins, lipids and drugs in human biopsy samples. The shared resource is supported by the CCSG, by research grants to individual investigators, and by significant institutional support from Vanderbilt.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Vanderbilt University Medical Center
United States
Zip Code
Gilchuk, Pavlo; Kuzmina, Natalia; Ilinykh, Philipp A et al. (2018) Multifunctional Pan-ebolavirus Antibody Recognizes a Site of Broad Vulnerability on the Ebolavirus Glycoprotein. Immunity 49:363-374.e10
Hebron, Katie E; Li, Elizabeth Y; Arnold Egloff, Shanna A et al. (2018) Alternative splicing of ALCAM enables tunable regulation of cell-cell adhesion through differential proteolysis. Sci Rep 8:3208
Bangaru, Sandhya; Zhang, Heng; Gilchuk, Iuliia M et al. (2018) A multifunctional human monoclonal neutralizing antibody that targets a unique conserved epitope on influenza HA. Nat Commun 9:2669
Means, Anna L; Freeman, Tanner J; Zhu, Jing et al. (2018) Epithelial Smad4 Deletion Up-Regulates Inflammation and Promotes Inflammation-Associated Cancer. Cell Mol Gastroenterol Hepatol 6:257-276
Du, Zhenfang; Lovly, Christine M (2018) Mechanisms of receptor tyrosine kinase activation in cancer. Mol Cancer 17:58
Zhang, Qin; Jeppesen, Dennis K; Higginbotham, James N et al. (2018) Mutant KRAS Exosomes Alter the Metabolic State of Recipient Colonic Epithelial Cells. Cell Mol Gastroenterol Hepatol 5:627-629.e6
Elion, David L; Cook, Rebecca S (2018) Harnessing RIG-I and intrinsic immunity in the tumor microenvironment for therapeutic cancer treatment. Oncotarget 9:29007-29017
Kook, Seunghyi; Qi, Aidong; Wang, Ping et al. (2018) Gene-edited MLE-15 Cells as a Model for the Hermansky-Pudlak Syndromes. Am J Respir Cell Mol Biol 58:566-574
Choksi, Yash A; Reddy, Vishruth K; Singh, Kshipra et al. (2018) BVES is required for maintenance of colonic epithelial integrity in experimental colitis by modifying intestinal permeability. Mucosal Immunol 11:1363-1374
Cooke, Allison L; Morris, Jamie; Melchior, John T et al. (2018) A thumbwheel mechanism for APOA1 activation of LCAT activity in HDL. J Lipid Res 59:1244-1255

Showing the most recent 10 out of 2462 publications