The overall goal of the Cell Imaging Shared Resource (CISR) is to supply Vanderbilt-lngram Cancer Center (VICC) researchers with access to cutting-edge technology and expert technical support for microscopic observation and analysis of tissue and cellular anatomy and physiology related to cancer research. The CISR maintains the full range of modern microscopy and digital imaging capabilities with the intent to enable and accelerate research. At the time of the last competitive renewal, this shared resource received an "Outstanding" rating. Optical microscopy can provide detailed morphological and quantitative information from whole animals and organs to organelles and even single molecules. In particular, fluorescence microscopy offers a high degree of specificity and sensitivity. Advanced techniques, such as confocal microscopy, fluorescence resonance energy transfer, total internal reflectance-excited fluorescence and two-photon-excited fluorescence, yield information with increasingly better spatial discrimination. The electron microscope is a versatile instrument for providing high-resolution structural information that is readily interpretable and easily quantified. From 2000- 2008, the CISR experienced more than 600% growth in both resources and usage. Since January 2009, the CISR has been directed by Sam Wells, Ph.D. (also managing director for Optical Microscopy) with Jay Jerome, Ph.D., as managing director of electron microscopy. David Piston, Ph.D., resource director from 1993- 2008, continues to serve as scientific director. He works with Drs. Wells and Jerome on overall strategic planning, helps interface with VICC leadership, and continues to serve as a mentor and resource for all shared resource personnel. The CISR service philosophy is embodied in four specific aims designed to assure the best use of resources by VICC members and facilitate the highest quality cancer research.
The specific aims are: Provide access to the full range of advanced, modern optical and electron microscopy; Furnish professional guidance in experimental design and data interpretation; Train users, on a continuing basis, in basic and advanced use of the equipment; Provide electron microscopy technical services for specimen preparation and sectioning.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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Vanderbilt University Medical Center
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Han, Ying; Signorello, Lisa B; Strom, Sara S et al. (2015) Generalizability of established prostate cancer risk variants in men of African ancestry. Int J Cancer 136:1210-7
Sanders, Melinda E; Schuyler, Peggy A; Simpson, Jean F et al. (2015) Continued observation of the natural history of low-grade ductal carcinoma in situ reaffirms proclivity for local recurrence even after more than 30 years of follow-up. Mod Pathol 28:662-9
Chaturvedi, R; de Sablet, T; Asim, M et al. (2015) Increased Helicobacter pylori-associated gastric cancer risk in the Andean region of Colombia is mediated by spermine oxidase. Oncogene 34:3429-40
Vilgelm, Anna E; Pawlikowski, Jeff S; Liu, Yan et al. (2015) Mdm2 and aurora kinase a inhibitors synergize to block melanoma growth by driving apoptosis and immune clearance of tumor cells. Cancer Res 75:181-93
Wei, Jinxiong; Noto, Jennifer M; Zaika, Elena et al. (2015) Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut 64:1040-8
Carrillo, A M; Bouska, A; Arrate, M P et al. (2015) Mdmx promotes genomic instability independent of p53 and Mdm2. Oncogene 34:846-56
Balko, Justin M; Giltnane, Jennifer M; Wang, Kai et al. (2014) Molecular profiling of the residual disease of triple-negative breast cancers after neoadjuvant chemotherapy identifies actionable therapeutic targets. Cancer Discov 4:232-45
Weeke, Peter; Mosley, Jonathan D; Hanna, David et al. (2014) Exome sequencing implicates an increased burden of rare potassium channel variants in the risk of drug-induced long QT interval syndrome. J Am Coll Cardiol 63:1430-7
Cheng, Feixiong; Jia, Peilin; Wang, Quan et al. (2014) Studying tumorigenesis through network evolution and somatic mutational perturbations in the cancer interactome. Mol Biol Evol 31:2156-69
Peng, Dun-Fa; Hu, Tian-Ling; Soutto, Mohammed et al. (2014) Loss of glutathione peroxidase 7 promotes TNF-?-induced NF-?B activation in Barrett's carcinogenesis. Carcinogenesis 35:1620-8

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