The overall goal of the Cell Imaging Shared Resource (CISR) is to supply Vanderbilt-lngram Cancer Center (VICC) researchers with access to cutting-edge technology and expert technical support for microscopic observation and analysis of tissue and cellular anatomy and physiology related to cancer research. The CISR maintains the full range of modern microscopy and digital imaging capabilities with the intent to enable and accelerate research. At the time of the last competitive renewal, this shared resource received an """"""""Outstanding"""""""" rating. Optical microscopy can provide detailed morphological and quantitative information from whole animals and organs to organelles and even single molecules. In particular, fluorescence microscopy offers a high degree of specificity and sensitivity. Advanced techniques, such as confocal microscopy, fluorescence resonance energy transfer, total internal reflectance-excited fluorescence and two-photon-excited fluorescence, yield information with increasingly better spatial discrimination. The electron microscope is a versatile instrument for providing high-resolution structural information that is readily interpretable and easily quantified. From 2000- 2008, the CISR experienced more than 600% growth in both resources and usage. Since January 2009, the CISR has been directed by Sam Wells, Ph.D. (also managing director for Optical Microscopy) with Jay Jerome, Ph.D., as managing director of electron microscopy. David Piston, Ph.D., resource director from 1993- 2008, continues to serve as scientific director. He works with Drs. Wells and Jerome on overall strategic planning, helps interface with VICC leadership, and continues to serve as a mentor and resource for all shared resource personnel. The CISR service philosophy is embodied in four specific aims designed to assure the best use of resources by VICC members and facilitate the highest quality cancer research.
The specific aims are: Provide access to the full range of advanced, modern optical and electron microscopy; Furnish professional guidance in experimental design and data interpretation; Train users, on a continuing basis, in basic and advanced use of the equipment; Provide electron microscopy technical services for specimen preparation and sectioning.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA068485-18
Application #
8733539
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
18
Fiscal Year
2014
Total Cost
$146,216
Indirect Cost
$89,303
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Paul, Pritha; Rellinger, Eric J; Qiao, Jingbo et al. (2017) Elevated TIMP-1 expression is associated with a prometastatic phenotype, disease relapse, and poor survival in neuroblastoma. Oncotarget 8:82609-82620
Stephenson, Jason R; Wang, Xiaohan; Perfitt, Tyler L et al. (2017) A Novel Human CAMK2A Mutation Disrupts Dendritic Morphology and Synaptic Transmission, and Causes ASD-Related Behaviors. J Neurosci 37:2216-2233
Bouziat, Romain; Hinterleitner, Reinhard; Brown, Judy J et al. (2017) Reovirus infection triggers inflammatory responses to dietary antigens and development of celiac disease. Science 356:44-50
Moyo, T K; Wilson, C S; Moore, D J et al. (2017) Myc enhances B-cell receptor signaling in precancerous B cells and confers resistance to Btk inhibition. Oncogene 36:4653-4661
Vilgelm, Anna E; Cobb, Priscilla; Malikayil, Kiran et al. (2017) MDM2 Antagonists Counteract Drug-Induced DNA Damage. EBioMedicine 24:43-55
LePage, Daniel P; Metcalf, Jason A; Bordenstein, Sarah R et al. (2017) Prophage WO genes recapitulate and enhance Wolbachia-induced cytoplasmic incompatibility. Nature 543:243-247
Li, Jun; Smith, Jarrod A; Dawson, Eric S et al. (2017) Optimized Translocator Protein Ligand for Optical Molecular Imaging and Screening. Bioconjug Chem 28:1016-1023
Zhang, Juliet; Weinrich, Jarret A P; Russ, Jeffrey B et al. (2017) A Role for Dystonia-Associated Genes in Spinal GABAergic Interneuron Circuitry. Cell Rep 21:666-678
Aune, T M; Crooke 3rd, P S; Patrick, A E et al. (2017) Expression of long non-coding RNAs in autoimmunity and linkage to enhancer function and autoimmune disease risk genetic variants. J Autoimmun 81:99-109
Esbenshade, Adam J; Zhao, Zhiguo; Aftandilian, Catherine et al. (2017) Multisite external validation of a risk prediction model for the diagnosis of blood stream infections in febrile pediatric oncology patients without severe neutropenia. Cancer 123:3781-3790

Showing the most recent 10 out of 2332 publications