Flow Cytometry is an important analytical and cell purification tool in cancer and immunological research. Modern cancer analysis requires innovative procedures that include both imaging methods and non-imaging analysis, such as that provided by flow cytometry. Flow cytometry allows the isolation of pure cell populations comprising less than 0.01% of a complex mixture. In addition, as an analytical tool it permits statistical analysis of cell population and cell function changes, apoptosis, intracellular protein expression, and gene transfection for example. Innovative methods that contribute to modern cancer research include gene expression detection with green fluorescent protein, parameter cytometry, apoptotic effect of chemotherapy, multi-drug resistance evaluation, and minimal residual disease detection. In addition, the integrated use of cell isolation (by flow cytometry) and downstream assessment of isolated cells (by proteomics or genomics) offers a powerful application of technologies offered by multiple Resources within the Knight Cancer Institute. High quality instrumentation is available to Knight Cancer Institute members in the form of two cell sorters and three analytical instruments. The Flow Cytometry Shared Resource provides consultation, training/teaching, technical expertise, and technical services in flow cytometry to meet the needs of Knight Cancer Institute members.
The Flow Cytometry Shared Resource offers state of the art equipment and support to Knight Cancer Institute researchers studying a wide array of human diseases. The resource positively impacts the study of hematopoietic and non-hematopoietic cancers, infectious diseases, inflammatory diseases, autoimmune diseases, and graft versus host disease;as well as stem cell biology and regenerative medicine.
|Le, T Domi; Carney, Patricia A; Lee-Lin, Frances et al. (2014) Differences in knowledge, attitudes, beliefs, and perceived risks regarding colorectal cancer screening among Chinese, Korean, and Vietnamese sub-groups. J Community Health 39:248-65|
|Ruffell, Brian; Chang-Strachan, Debbie; Chan, Vivien et al. (2014) Macrophage IL-10 blocks CD8+ T cell-dependent responses to chemotherapy by suppressing IL-12 expression in intratumoral dendritic cells. Cancer Cell 26:623-37|
|Martin, Jessica L; Yates, Phillip A; Soysa, Radika et al. (2014) Metabolic reprogramming during purine stress in the protozoan pathogen Leishmania donovani. PLoS Pathog 10:e1003938|
|Hitzemann, Robert; Bottomly, Daniel; Iancu, Ovidiu et al. (2014) The genetics of gene expression in complex mouse crosses as a tool to study the molecular underpinnings of behavior traits. Mamm Genome 25:12-22|
|Liu, Betty Y; O'Malley, Jean; Mori, Motomi et al. (2014) The association of type and number of chronic diseases with breast, cervical, and colorectal cancer screening. J Am Board Fam Med 27:669-81|
|Affara, Nesrine I; Ruffell, Brian; Medler, Terry R et al. (2014) B cells regulate macrophage phenotype and response to chemotherapy in squamous carcinomas. Cancer Cell 25:809-21|
|Cope, Leslie M; Fackler, Mary Jo; Lopez-Bujanda, Zoila et al. (2014) Do breast cancer cell lines provide a relevant model of the patient tumor methylome? PLoS One 9:e105545|
|Caputo, Nicholas; Jackson, Melanie A; Castle, Jessica R et al. (2014) Biochemical stabilization of glucagon at alkaline pH. Diabetes Technol Ther 16:747-58|
|Yao, Huilan; Goldman, Devorah C; Nechiporuk, Tamilla et al. (2014) Corepressor Rcor1 is essential for murine erythropoiesis. Blood 123:3175-84|
|Davare, Monika A; Lal, Sangeet; Peckham, Jennifer L et al. (2014) Secreted meningeal chemokines, but not VEGFA, modulate the migratory properties of medulloblastoma cells. Biochem Biophys Res Commun 450:555-60|
Showing the most recent 10 out of 88 publications