The Analytic Microscopy Core Facility (AMC) was established in 1999 to provide investigators with the tools and technical expertise required for acquisition and analysis of advanced optical microscopy applications. Optical microscopy offers investigators the ability to visualize and quantify complex cellular and sub-cellular level processes in multiple dimensions. The core's goal is to provide expert training, experimental planning, hands-on assistance, coupled with state-of-the-art instrumentation and responsible equipment maintenance. AMC is well equipped, skillfully staffed, heavily used and has proven to be an invaluable resource for Cancer Center researchers interested in the most demanding projects. The core seeks to continue to identify and obtain emerging technologies that meet the needs of Moffitt Members. Since the last renewal, the AMC has continued to experience a dramatic increase in usage, which has prompted the growth of services, instrumentation, and expert personnel. Since 2006, the Core has added five major instrument systems and upgraded four older existing systems. New equipment include Aperio's ScanScope XT slide scanning technology to include a higher throughput and more advanced analysis system also capable of fluorescent imaging;a state-of-the-art Leica SPS AOBS tandem laser scanning confocal microscope;a fully automated Zeiss Observer wide-field fluorescence inverted microscope;and a cluster of stand alone offline image analysis workstations. In addition to added instrumentation, the AMC hired an additional Microscopy Specialist 111 (Joseph Johnson in 2006) to supplement the Core Staff Scientist (Mark Lloyd in 2003) and Microscopy Specialist II (Nancy Burke in 2005). The Core requests CCSG Support of $109,212, which is 34% of its operational budget. Over 70% of usage is by Moffitt members and peer-reviewed cancer investigators.

Public Health Relevance

The Analytic Microscopy Core is responsive to the science performed at the Cancer Center and committed to the acquisition of state-of-the-art instrumentation that is easy to use and that expands microscopy capabilities to meet the needs of Moffitt's members. This approach allows members to focus on their science and leaves the expertise, maintenance, and support of high-end microscopy equipment to a highly trained and dedicated full-time staff.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA076292-16
Application #
8613449
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2014-02-01
Budget End
2015-01-31
Support Year
16
Fiscal Year
2014
Total Cost
$42,240
Indirect Cost
$17,172
Name
H. Lee Moffitt Cancer Center & Research Institute
Department
Type
DUNS #
139301956
City
Tampa
State
FL
Country
United States
Zip Code
33612
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