The Embryonic Stem Cell (ESC) Core is responsible for: Creating and providing state-of-the-art reagents for the production of targeted mutations in murine embryonic stem cells. Creating and maintaining quality-controlled embryonic stem cell lines from a variety of mouse backgrounds. Teaching the methodology of embryonic stem cell culture and manipulation. The ESC Core provides consultative services for Siteman Cancer Center members when they are designing knock-out or knock-in vectors, and it creates and distributes user-friendly plasmids that facilitate the generation of targeting vectors. By providing a comprehensive service, the ESC Core facilitates the production of gain-offunction and loss-of-function mouse models for Siteman Cancer Center faculty.

Public Health Relevance

This core assists investigators in the Cancer Center with the generation of reagents used to make sophisticated mouse models of human diseases, including cancer. These reagents help us to understand how individual genes work, and how mutations work to cause cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA091842-11
Application #
8380301
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
11
Fiscal Year
2012
Total Cost
$143,764
Indirect Cost
$55,503
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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