The Embryonic Stem Cell (ESC) Core is responsible for: Creating and providing state-of-the-art reagents for the production of targeted mutations in murine embryonic stem cells. Creating and maintaining quality-controlled embryonic stem cell lines from a variety of mouse backgrounds. Teaching the methodology of embryonic stem cell culture and manipulation. The ESC Core provides consultative services for Siteman Cancer Center members when they are designing knock-out or knock-in vectors, and it creates and distributes user-friendly plasmids that facilitate the generation of targeting vectors. By providing a comprehensive service, the ESC Core facilitates the production of gain-offunction and loss-of-function mouse models for Siteman Cancer Center faculty.

Public Health Relevance

This core assists investigators in the Cancer Center with the generation of reagents used to make sophisticated mouse models of human diseases, including cancer. These reagents help us to understand how individual genes work, and how mutations work to cause cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA091842-11
Application #
8380301
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
11
Fiscal Year
2012
Total Cost
$143,764
Indirect Cost
$55,503
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Waqar, Saiama N; Waqar, Sadaf H; Trinkaus, Kathryn et al. (2018) Brain Metastases at Presentation in Patients With Non-Small Cell Lung Cancer. Am J Clin Oncol 41:36-40
May-Zhang, Aaron A; Deal, Karen K; Southard-Smith, E Michelle (2018) Optimization of Laser-Capture Microdissection for the Isolation of Enteric Ganglia from Fresh-Frozen Human Tissue. J Vis Exp :
Harris-Hayes, Marcie; Steger-May, Karen; van Dillen, Linda R et al. (2018) Reduced Hip Adduction Is Associated With Improved Function After Movement-Pattern Training in Young People With Chronic Hip Joint Pain. J Orthop Sports Phys Ther 48:316-324
Beleckas, Casey M; Gerull, William; Wright, Melissa et al. (2018) Variability of PROMIS Scores Across Hand Conditions. J Hand Surg Am :
Prudner, Bethany Cheree; Sun, Fangdi; Kremer, Jeffrey Charles et al. (2018) Amino Acid Uptake Measured by [18F]AFETP Increases in Response to Arginine Starvation in ASS1-Deficient Sarcomas. Theranostics 8:2107-2116
Mundt, Filip; Rajput, Sandeep; Li, Shunqiang et al. (2018) Mass Spectrometry-Based Proteomics Reveals Potential Roles of NEK9 and MAP2K4 in Resistance to PI3K Inhibition in Triple-Negative Breast Cancers. Cancer Res 78:2732-2746
Burclaff, Joseph; Mills, Jason C (2018) Plasticity of differentiated cells in wound repair and tumorigenesis, part I: stomach and pancreas. Dis Model Mech 11:
Meinerz, Kelsey; Beeman, Scott C; Duan, Chong et al. (2018) Bayesian Modeling of NMR Data: Quantifying Longitudinal Relaxation in Vivo, and in Vitro with a Tissue-Water-Relaxation Mimic (Crosslinked Bovine Serum Albumin). Appl Magn Reson 49:3-24
Rocha, Agostinho G; Franco, Antonietta; Krezel, Andrzej M et al. (2018) MFN2 agonists reverse mitochondrial defects in preclinical models of Charcot-Marie-Tooth disease type 2A. Science 360:336-341
Lin, Jonathan B; Sene, Abdoulaye; Santeford, Andrea et al. (2018) Oxysterol Signatures Distinguish Age-Related Macular Degeneration from Physiologic Aging. EBioMedicine 32:9-20

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