The Siteman Cancer Center Molecular and Genomic Analysis (MGA) Core represents the physical and programmatic integration of two previous cores, the Multiplexed Gene Analysis (GeneChip) Core and the Molecular Core. The unified vision and specific aims of this core facility are: 1) To provide members with facilitated and cost-effective access to high complexity genomic analysis platforms including Affymetrix GeneChipd) microarray technology for whole genome analysis of gene transcription, genotyping, genome copy number, and DNA sequence, as well as PCR-based directed DNA resequencing and quantitative PCR instrumentation, and;2) To provide a clinically accredited laboratory environment for developing, validating, and translating genome-based biomarker assays toward their use in clinical trials and ultimately, routine patient management. To accomplish these aims, the Core continues to provide Affymetrix microarray services to investigators. All array platforms are supported for human and many other model organism species, including custom genotyping arrays that implement four-color, multiplexed genotyping assays using Molecular Inversion Probe (MIP) chemistry. New RNA amplification protocols have been validated for expression profiling from limiting amounts of clinical material (such as flow sorted cell populations or microdissected tissue) as well paraffin embedded tissues. A reorganization of operations and the conduct of CLIA-Iicensed clinical testing have promoted a new level of quality control and proficiency testing associated with assays conducted even for purely research purposes. New staff and additional faculty oversight also allow for consultation with investigators for 'CLIA-grade'assay development and interpretation in the context of clinical and translational studies. Like its predecessors, the new MGA Core maintains increasingly close integration with the Tissue Procurement Core and the Bioinformatics Core, to provide a seamless conduit for the collection, analysis, and interpretation of genomic data generated from clinical and experimental biospecimens.
The Siteman Cancer Center Molecular and Genomic Analysis (MGA) Core allows cancer scientists to perform complex and expensive genome studies that would not othen/vise be possible in their own laboratories. The data generated by use of this facility is helping researchers to better understand the genetic basis of cancer which, in turn, will lead to new approaches for cancer treatment and prevention.
|Zuiani, Adam; Chen, Kevin; Schwarz, Megan C et al. (2016) A Library of Infectious Hepatitis C Viruses with Engineered Mutations in the E2 Gene Reveals Growth-Adaptive Mutations That Modulate Interactions with Scavenger Receptor Class B Type I. J Virol 90:10499-10512|
|Abboud, Ramzi; Keller, Jesse; Slade, Michael et al. (2016) Severe Cytokine-Release Syndrome after T Cell-Replete Peripheral Blood Haploidentical Donor Transplantation Is Associated with Poor Survival and Anti-IL-6 Therapy Is Safe and Well Tolerated. Biol Blood Marrow Transplant 22:1851-60|
|Johnson, Kimberly J; Zoellner, Nancy L; Gutmann, David H (2016) Peri-gestational risk factors for pediatric brain tumors in Neurofibromatosis Type 1. Cancer Epidemiol 42:53-9|
|Brownson, Ross C; Dodson, Elizabeth A; Kerner, Jon F et al. (2016) Framing research for state policymakers who place a priority on cancer. Cancer Causes Control 27:1035-41|
|Chou, Chun; Verbaro, Daniel J; Tonc, Elena et al. (2016) The Transcription Factor AP4 Mediates Resolution of Chronic Viral Infection through Amplification of Germinal Center B Cell Responses. Immunity 45:570-82|
|Durai, Vivek; Murphy, Kenneth M (2016) Functions of Murine Dendritic Cells. Immunity 45:719-736|
|Beeman, Scott C; Shui, Ying-Bo; Perez-Torres, Carlos J et al. (2016) O2 -sensitive MRI distinguishes brain tumor versus radiation necrosis in murine models. Magn Reson Med 75:2442-7|
|Mertins, Philipp; Mani, D R; Ruggles, Kelly V et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534:55-62|
|Niu, Haixia; Hadwiger, Gayla; Fujiwara, Hideji et al. (2016) Pathways of retinoid synthesis in mouse macrophages and bone marrow cells. J Leukoc Biol 99:797-810|
|Willet, Spencer G; Mills, Jason C (2016) Stomach Organ and Cell Lineage Differentiation: from Embryogenesis to Adult Homeostasis. Cell Mol Gastroenterol Hepatol 2:546-559|
Showing the most recent 10 out of 947 publications