The Bioscience Screening Facility (BSF) Shared Resource was first established in 2003 by the Department of Chemical &Systems Biology in the School of Medicine. The BSF's mission is to provide researchers at Stanford with ability to run high-throughput chemical, siRNA, cDNA, and high-content screens for the purpose of drug and/or target discovery. The BSF brings research approaches that were previously carried out exclusively in industry into academia to advance basic biomedical research. This high-throughput screening (HTS) laboratory allows researchers to discover novel modulators of targets that are of interest to academic investigators. The center incorporates instrumentation, databases, compound libraries, and personnel whose previous sole domains were in industry. Among our instrumentation are a Molecular Devices ImageXpress Micro fluorescence microplate imager with live cell option, a Caliper Life Sciences SciClone ALH3000 microplate liquid handler and the Molecular Devices Analyst GT and FlexStation II 384 fluorescence, luminescence and absorbance microplate readers. The facility has over 130,000 small molecules for compound screens, 15,000 cDNAs for genomic screens, and the siARRAY whole human genome siRNA (small interfering RNA) library from ThermoFisher Scientific (formerly Dharmacon) targeting 21,000 genes. Access to this equipment and services are provided much more cost-effectively and efficiently through this centralized facility. Before this facility was available, most of these screening experiments were too expensive to be performed at Stanford. This Cancer Center Shared Resource, now provides the means for Cancer Center members to perform high-throughput screens and use high-throughput equipment, such as plate readers, for their research. Additionally, it enables investigators to discover novel cancer targets and potential therapeutics. The Shared Resource makes every effort to bring in new technologies, such as whole genome siRNA knockdown screens, and other services to meet the evolving needs of Cancer Center members. Although the BSF has been a Cancer Center Shared Resource only since 2007, investigators in nine Programs of the Cancer Center have used the BSF equipment and services for their studies.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA124435-06
Application #
8375600
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-06-01
Budget End
2013-05-31
Support Year
6
Fiscal Year
2012
Total Cost
$57,876
Indirect Cost
$4
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Lee, Bee L; Fan, Shenghua K; Lu, Ying (2017) A curve-free Bayesian decision-theoretic design for two-agent Phase I trials. J Biopharm Stat 27:34-43
Mohanty, Suchismita; Chen, Zixin; Li, Kai et al. (2017) A Novel Theranostic Strategy for MMP-14-Expressing Glioblastomas Impacts Survival. Mol Cancer Ther 16:1909-1921
Alcántara-Hernández, Marcela; Leylek, Rebecca; Wagar, Lisa E et al. (2017) High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization. Immunity 47:1037-1050.e6
Chao, Mark P; Gentles, Andrew J; Chatterjee, Susmita et al. (2017) Human AML-iPSCs Reacquire Leukemic Properties after Differentiation and Model Clonal Variation of Disease. Cell Stem Cell 20:329-344.e7
Rogers, Zoë N; McFarland, Christopher D; Winters, Ian P et al. (2017) A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivo. Nat Methods 14:737-742
Sun, Ruping; Hu, Zheng; Sottoriva, Andrea et al. (2017) Between-region genetic divergence reflects the mode and tempo of tumor evolution. Nat Genet 49:1015-1024
Jin, Yuxue; Lai, Tze Leung (2017) A new approach to regression analysis of censored competing-risks data. Lifetime Data Anal 23:605-625
Clarke, Christina A; Glaser, Sally L; Leung, Rita et al. (2017) Prevalence and characteristics of cancer patients receiving care from single vs. multiple institutions. Cancer Epidemiol 46:27-33
Han, Summer S; Ten Haaf, Kevin; Hazelton, William D et al. (2017) The impact of overdiagnosis on the selection of efficient lung cancer screening strategies. Int J Cancer 140:2436-2443
Wender, Paul A; Hardman, Clayton T; Ho, Stephen et al. (2017) Scalable synthesis of bryostatin 1 and analogs, adjuvant leads against latent HIV. Science 358:218-223

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