The Genomic Profiling Shared Resource (GPSR) of the Dan L. Duncan Cancer Center (DLDCC) at Baylor College of Medicine (BCM) utilizes the expertise available at both BCM and Texas Children's Hospital (TCH) to offer a comprehensive suite of services using cutting edge genomic and transcriptomic technologies to DLDCC members. The GPSR core combines cutting edge technologies to provide state-of-the-art quality microarray-based and next generation sequencing-based services and analyses for both transcriptional and genomic profiling. This resource provides assistance to DLDCC researchers in utilizing microarray technology, next generation sequencing technology, good experimental design, and data management and data analysis resources. We will begin offering next generation sequencing technology (lllumina Genome Analyzer II) to DLDCC members in October 2009. Many DLDCC researchers are interested in utilizing state-of-the-art technologies such as microarray expression profiling to attempt to dissect the causes and effects associated with cancer. For individual laboratories, the costs and levels of expertise associated with establishing a microarray capability is prohibitive (initial equipment purchases can cost between $250,000 and $750,000) requiring a facility like the GPSR. Within the past decade we have witnessed significant advancements in research that are directly associated with the output of the genome sequencing endeavor. The results of these achievements provide hope to investigators researching complex disease including cancer. In cancer, complex barriers to the identification of cause include not only chromosomal abnormalities (gross and submicroscopic) but alterations in one or several genes having aberrant expression profiles or even hundreds to thousands of genes with perturbed expression. This can result in a mishmash of cancer gene expression profiles that is difficult to sort through presenting a challenge to researchers attempting to elucidate the cause and effect of cancer. The GPSR works to provide DLDCC members a solid base of expertise to tap in order to make sense of the large data sets generated with this technology.
Cancer is a complex disease and researchers who endeavor to dissect the causes associated with cancer are now able to delve deeper than ever before utilizing tools that aid in the analyses of genomic and transcriptomic changes associated with a cancer state. This shared resource provides an avenue for these researchers to access both these cutting edge technologies and the expertise necessary to successfully use them.
|Addison, Joseph B; Koontz, Colton; Fugett, James H et al. (2015) KAP1 promotes proliferation and metastatic progression of breast cancer cells. Cancer Res 75:344-55|
|Torbit, Lindsey A; Albiani, Jenna J; Crangle, Cassandra J et al. (2015) Fear of recurrence: the importance of self-efficacy and satisfaction with care in gay men with prostate cancer. Psychooncology 24:691-8|
|Thrift, Aaron P; Garcia, Jose M; El-Serag, Hashem B (2014) A multibiomarker risk score helps predict risk for Barrett's esophagus. Clin Gastroenterol Hepatol 12:1267-71|
|Bhattacharya, Abhisek; Parillon, Xyanthine; Zeng, Shenyan et al. (2014) Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis. J Biol Chem 289:26525-32|
|Ramasamy, Ranjith; Ridgeway, Alex; Lipshultz, Larry I et al. (2014) Integrative DNA methylation and gene expression analysis identifies discoidin domain receptor 1 association with idiopathic nonobstructive azoospermia. Fertil Steril 102:968-973.e3|
|Kowalkowski, Marc A; Day, Rena S; Du, Xianglin L et al. (2014) Cumulative HIV viremia and non-AIDS-defining malignancies among a sample of HIV-infected male veterans. J Acquir Immune Defic Syndr 67:204-11|
|Geldres, Claudia; Savoldo, Barbara; Hoyos, Valentina et al. (2014) T lymphocytes redirected against the chondroitin sulfate proteoglycan-4 control the growth of multiple solid tumors both in vitro and in vivo. Clin Cancer Res 20:962-71|
|Young, Evelin; Zheng, Ze-Yi; Wilkins, Angela D et al. (2014) Regulation of Ras localization and cell transformation by evolutionarily conserved palmitoyltransferases. Mol Cell Biol 34:374-85|
|Anurathapan, Usanarat; Leen, Ann M; Brenner, Malcolm K et al. (2014) Engineered T cells for cancer treatment. Cytotherapy 16:713-33|
|Thrift, Aaron P; Kramer, Jennifer R; Alsarraj, Abeer et al. (2014) Fat mass by bioelectrical impedance analysis is not associated with increased risk of Barrett esophagus. J Clin Gastroenterol 48:218-23|
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