The Structural Biology Shared Sen/ice (SBSS) helps researchers use the unique information derived from macromolecular structures to understand the molecular basis of cancer-causing cellular defects and to design drugs that mitigate such defects. SBSS comprises the X-ray Crystallography Shared Service (XRSS) and Nuclear Magnetic Resonance Shared Service (NMRSS). Together, these two facilities offer different technical approaches that work in synergy to allow researchers at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) to view cancer-related biological macromolecules at the molecular level. Researchers from UMGCC account for more than one-half of the use of SBSS. Drs. David Weber and Eric Toth advise UMGCC investigators on how best to use SBSS to advance the goals of their project. To aid in this process, the SBSS has adopted an experimental pipeline that allows UMGCC investigators to determine rapidly which experimental technique best suits their needs and proceed with the experimental design that provides the most useful data in the shortest amount of time. The x-ray component of SBSS gives UMGCC investigators access to an Oryx Nano crystallization robot and a powerful Rigaku data collection system. These instruments provide UMGCC investigators with the tools to produce rapidly diffraction-quality crystals of cancer-related macromolecules and determine their three-dimensional structures. The NMR component ofthe SBSS currently provides access to both 600- and 800-MHz spectrometers. By April 2011, the NMR facility will acquire and install a 950-MHz spectrometer, the only such instrument at an academic institution in the United States. This array of cutting-edge spectrometers will allow UMGCC researchers to access the most sophisticated techniques for determining solution structures and examining the dynamics of large cancer-related macromolecules. Using these technologies, SBSS has supplied a number of UMGCC researchers with key experimental data that greatly enhanced their research programs.
Aberrant changes that cause cancer involve molecular events governed by both local protein conformations and global macromolecular architectures. Understanding the structural basis of these events accelerates the design of therapeutic interventions. SBSS provides NMR and x-ray crystallography capabilities to UMGCC researchers as a means to advance both basic research and translational efforts to combat cancer.
|Wang, Lei; Ma, Xiao; Xu, Xiaolei et al. (2017) Systematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafish. Sci Rep 7:1250|
|Nowak, Rebecca G; Ambulos, Nicholas P; Schumaker, Lisa M et al. (2017) Genotyping of high-risk anal human papillomavirus (HPV): ion torrent-next generation sequencing vs. linear array. Virol J 14:112|
|Thomas, Stefani N; Yang, Austin J (2017) Mass Spectrometry Analysis of Lysine Posttranslational Modifications of Tau Protein from Alzheimer's Disease Brain. Methods Mol Biol 1523:161-177|
|Kaczanowska, Sabina; Joseph, Ann Mary; Guo, Jitao et al. (2017) A Synthetic CD8?:MyD88 Coreceptor Enhances CD8+ T-cell Responses to Weakly Immunogenic and Lowly Expressed Tumor Antigens. Cancer Res 77:7049-7058|
|Larrosa-Garcia, Maria; Baer, Maria R (2017) FLT3 Inhibitors in Acute Myeloid Leukemia: Current Status and Future Directions. Mol Cancer Ther 16:991-1001|
|Stick, Line B; Yu, Jen; Maraldo, Maja V et al. (2017) Joint Estimation of Cardiac Toxicity and Recurrence Risks After Comprehensive Nodal Photon Versus Proton Therapy for Breast Cancer. Int J Radiat Oncol Biol Phys 97:754-761|
|Connolly, Nina P; Stokum, Jesse A; Schneider, Craig S et al. (2017) Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. PLoS One 12:e0174557|
|Carter-Cooper, Brandon A; Fletcher, Steven; Ferraris, Dana et al. (2017) Synthesis, characterization and antineoplastic activity of bis-aziridinyl dimeric naphthoquinone - A novel class of compounds with potent activity against acute myeloid leukemia cells. Bioorg Med Chem Lett 27:6-10|
|Young, Christina A; Rorke, Ellen A; Adhikary, Gautam et al. (2017) Loss of epidermal AP1 transcription factor function reduces filaggrin level, alters chemokine expression and produces an ichthyosis-related phenotype. Cell Death Dis 8:e2840|
|Nowak, Rebecca G; Bentzen, Søren M; Ravel, Jacques et al. (2017) Rectal microbiota among HIV-uninfected, untreated HIV, and treated HIV-infected in Nigeria. AIDS 31:857-862|
Showing the most recent 10 out of 239 publications