The Structural Biology Shared Sen/ice (SBSS) helps researchers use the unique information derived from macromolecular structures to understand the molecular basis of cancer-causing cellular defects and to design drugs that mitigate such defects. SBSS comprises the X-ray Crystallography Shared Service (XRSS) and Nuclear Magnetic Resonance Shared Service (NMRSS). Together, these two facilities offer different technical approaches that work in synergy to allow researchers at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) to view cancer-related biological macromolecules at the molecular level. Researchers from UMGCC account for more than one-half of the use of SBSS. Drs. David Weber and Eric Toth advise UMGCC investigators on how best to use SBSS to advance the goals of their project. To aid in this process, the SBSS has adopted an experimental pipeline that allows UMGCC investigators to determine rapidly which experimental technique best suits their needs and proceed with the experimental design that provides the most useful data in the shortest amount of time. The x-ray component of SBSS gives UMGCC investigators access to an Oryx Nano crystallization robot and a powerful Rigaku data collection system. These instruments provide UMGCC investigators with the tools to produce rapidly diffraction-quality crystals of cancer-related macromolecules and determine their three-dimensional structures. The NMR component ofthe SBSS currently provides access to both 600- and 800-MHz spectrometers. By April 2011, the NMR facility will acquire and install a 950-MHz spectrometer, the only such instrument at an academic institution in the United States. This array of cutting-edge spectrometers will allow UMGCC researchers to access the most sophisticated techniques for determining solution structures and examining the dynamics of large cancer-related macromolecules. Using these technologies, SBSS has supplied a number of UMGCC researchers with key experimental data that greatly enhanced their research programs.

Public Health Relevance

Aberrant changes that cause cancer involve molecular events governed by both local protein conformations and global macromolecular architectures. Understanding the structural basis of these events accelerates the design of therapeutic interventions. SBSS provides NMR and x-ray crystallography capabilities to UMGCC researchers as a means to advance both basic research and translational efforts to combat cancer.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Maryland Baltimore
United States
Zip Code
Nygård, Lotte; Vogelius, Ivan R; Fischer, Barbara M et al. (2016) Early lesion-specific (18)F-FDG PET response to chemotherapy predicts time to lesion progression in locally advanced non-small cell lung cancer. Radiother Oncol 118:460-4
Fox, Jennifer M; Moynihan, James R; Mott, Bryan T et al. (2016) Artemisinin-derived dimer ART-838 potently inhibited human acute leukemias, persisted in vivo, and synergized with antileukemic drugs. Oncotarget 7:7268-79
Joseph, Ann Mary; Srivastava, Ratika; Zabaleta, Jovanny et al. (2016) Cross-talk between 4-1BB and TLR1-TLR2 Signaling in CD8+ T Cells Regulates TLR2's Costimulatory Effects. Cancer Immunol Res 4:708-16
Zandberg, Dan P; Liu, Sandy; Goloubeva, Olga et al. (2016) Oropharyngeal cancer as a driver of racial outcome disparities in squamous cell carcinoma of the head and neck: 10-year experience at the University of Maryland Greenebaum Cancer Center. Head Neck 38:564-72
Doshi, Kshama A; Trotta, Rossana; Natarajan, Karthika et al. (2016) Pim kinase inhibition sensitizes FLT3-ITD acute myeloid leukemia cells to topoisomerase 2 inhibitors through increased DNA damage and oxidative stress. Oncotarget 7:48280-48295
Ambulos Jr, Nicholas P; Schumaker, Lisa M; Mathias, Trevor J et al. (2016) Next-Generation Sequencing-Based HPV Genotyping Assay Validated in Formalin-Fixed, Paraffin-Embedded Oropharyngeal and Cervical Cancer Specimens. J Biomol Tech 27:46-52
Temburnikar, Kartik W; Ross, Christina R; Wilson, Gerald M et al. (2015) Antiproliferative activities of halogenated pyrrolo[3,2-d]pyrimidines. Bioorg Med Chem 23:4354-63
Kochel, Tyler J; Fulton, Amy M (2015) Multiple drug resistance-associated protein 4 (MRP4), prostaglandin transporter (PGT), and 15-hydroxyprostaglandin dehydrogenase (15-PGDH) as determinants of PGE2 levels in cancer. Prostaglandins Other Lipid Mediat 116-117:99-103
Rasmussen, Jacob H; Vogelius, Ivan R; Aznar, Marianne C et al. (2015) Spatio-temporal stability of pre-treatment 18F-Fludeoxyglucose uptake in head and neck squamous cell carcinomas sufficient for dose painting. Acta Oncol 54:1416-22
Shih, Yu-Huan; Zhang, Yuji; Ding, Yonghe et al. (2015) Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish. Circ Cardiovasc Genet 8:261-9

Showing the most recent 10 out of 207 publications