The Hollings Cancer Center (HCC) Flow Cytometry &Cell Sorting (FCCS) Shared Resource provides HCC members comprehensive analytic flow cytometry and state-of-the-art high speed cell sorting services in a cost effective manner. Under the directorship of Daohong Zhou, MD, the FCCS is equipped with a Becton- Dickinson FACSCalibur Analytical Flow Cytometer, a Becton-Dickinson FACSAria Cell Sorter and a Dako VIoFlo High-Speed Cell Sorter. The staff of the resource have considerable expertise in high-speed sorting of rare populations of cells including stem/progenitor cells and epitope-specific T cells and are continually expanding the repertoire of available techniques to meet the needs of the innovative research base within the HCC. Since 2003, the HCC FCCS Shared Resource has supported research that has resulted in 123 publications by 33 investigators representing all of the HCC programs and eight Departments at the Medical University of South Carolina. The HCC FCCS Shared Resource has played an essential role in several of the seminal discoveries made by members of the HCC during this time. The specific objectives of the HCC FCCS Shared Resource are to: ? Provide multi-parametric and multi-laser measurements including measurements of: apoptosis, cell proliferation, cell cycle distribution, and immunophenotyping, as well as detection of intracellular proteins, membrane perturbations, and bioactive molecules ? Provide high-speed cell sorting based on cell surface marker immunostaining and/or side-population staining, and expression of green fluorescent protein ? Provide consultation and assistance concerning experimental design, sample preparation, data analysis, and preparation of high quality figures for publications and grant applications as they relate to flow cytometry ? Provide training in the use of routine flow cytometric technology and offer more advanced training for HCC members concerning the newly developed flow cytometric assays ? Identify and develop new flow cytometric technologies to assist cutting-edge research in the HCC

Public Health Relevance

The Flow Cytometry &Cell Sorting Shared Resource at the Hollings Cancer Center of the Medical University of South Carolina provides essential research support services to enhance scientific innovation and productivity in a cost efficient manner.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA138313-05
Application #
8456107
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-04-01
Budget End
2014-03-31
Support Year
5
Fiscal Year
2013
Total Cost
$86,184
Indirect Cost
$27,754
Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Zunke, Friederike; Moise, Alexandra C; Belur, Nandkishore R et al. (2018) Reversible Conformational Conversion of ?-Synuclein into Toxic Assemblies by Glucosylceramide. Neuron 97:92-107.e10
Schutt, Steven D; Wu, Yongxia; Tang, Chih-Hang Anthony et al. (2018) Inhibition of the IRE-1?/XBP-1 pathway prevents chronic GVHD and preserves the GVL effect in mice. Blood Adv 2:414-427
Cartmell, Kathleen B; Mzik, Carlie R; Sundstrom, Beth L et al. (2018) HPV Vaccination Communication Messages, Messengers, and Messaging Strategies. J Cancer Educ :
King, Bill; Ndoen, Enjelita; Borland, Ron (2018) Smokers' risk perceptions and misperceptions of cigarettes, e-cigarettes and nicotine replacement therapies. Drug Alcohol Rev 37:810-817
Lin, Ching Ying; Kwon, Hyunwoo; Rangel Rivera, Guillermo O et al. (2018) Sex Differences in Using Systemic Inflammatory Markers to Prognosticate Patients with Head and Neck Squamous Cell Carcinoma. Cancer Epidemiol Biomarkers Prev 27:1176-1185
Cartmell, Kathleen B; Young-Pierce, Jennifer; McGue, Shannon et al. (2018) Barriers, facilitators, and potential strategies for increasing HPV vaccination: A statewide assessment to inform action. Papillomavirus Res 5:21-31
Alawieh, Ali; Andersen, Meredith; Adkins, DeAnna L et al. (2018) Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection. J Neurosci 38:6527-6545
Noble, Kenyaria V; Reyzer, Michelle L; Barth, Jeremy L et al. (2018) Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury. Front Mol Neurosci 11:243
Janz, Tyler A; Kim, Joanne; Hill, Elizabeth G et al. (2018) Association of Care Processes With Timely, Equitable Postoperative Radiotherapy in Patients With Surgically Treated Head and Neck Squamous Cell Carcinoma. JAMA Otolaryngol Head Neck Surg :
Graboyes, Evan M; Ellis, Mark A; Li, Hong et al. (2018) Racial and Ethnic Disparities in Travel for Head and Neck Cancer Treatment and the Impact of Travel Distance on Survival. Cancer 124:3181-3191

Showing the most recent 10 out of 536 publications