The Molecular Core will provide several critical services to promote research in drug abuse research. First, we provide a wide range of vector design, construction, and expression services to investigators who need to study the function of a given gene product. This includes epitope-tagged vectors to monitor protein trafficking, or to assess protein-protein interactions. We also provide viral transduction vector design and construction for those investigators who are interested in expression of genes in either primary cells or a cell line. We also provide a wide variety of services for those investigators who are interested in studying the regulation of gene expression. This includes assessment of transcription regulation at the level of gene promoter mapping and function, as well as measurement of mRNA expression by a variety of techniques including quantitative RT-PCR, northern blot analysis, and RNase protection analysis. Finally, we provide genotyping services to the Animal Core, a service which is critical for those strains that must be maintained by breeding heterozygous breeding pairs. In summary, our services are extremely helpful to investigators who are either experienced or inexperienced in molecular biology. It is also apparent that our Core has served to promote interactions and build collaborations between investigators with interests in drug abuse research.
The Molecular Core provides a wide variety of services to investigators interested in drug abuse research, and involved in studies at the molecular level. This Core provides necessary genotyping support for the Animal Core. We provide valuable assistance within Temple University, and to the wider scientific community, and the benefits of our services also include the stimulation of collaborative research efforts.
|Console-Bram, Linda; Brailoiu, Eugen; Brailoiu, Gabriela Cristina et al. (2014) Activation of GPR18 by cannabinoid compounds: a tale of biased agonism. Br J Pharmacol 171:3908-17|
|Miller, Jonathan S; Barr, Jeffrey L; Harper, Lauren J et al. (2014) The GSK3 signaling pathway is activated by cocaine and is critical for cocaine conditioned reward in mice. PLoS One 9:e88026|
|Zhao, Pingwei; Lane, Tom R; Gao, Helen G L et al. (2014) Crucial positively charged residues for ligand activation of the GPR35 receptor. J Biol Chem 289:3625-38|
|Chatila, W M; Criner, G J; Hancock, W W et al. (2014) Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers. Clin Exp Immunol 177:341-52|
|Enman, Nicole M; Zhang, Yong; Unterwald, Ellen M (2014) Connecting the pathology of posttraumatic stress and substance use disorders: monoamines and neuropeptides. Pharmacol Biochem Behav 117:61-9|
|Shi, Xiangdang; Miller, Jonathan S; Harper, Lauren J et al. (2014) Reactivation of cocaine reward memory engages the Akt/GSK3/mTOR signaling pathway and can be disrupted by GSK3 inhibition. Psychopharmacology (Berl) 231:3109-18|
|Kong, Weimin; Li, Hongbo; Tuma, Ronald F et al. (2014) Selective CB2 receptor activation ameliorates EAE by reducing Th17 differentiation and immune cell accumulation in the CNS. Cell Immunol 287:1-17|
|Dimattio, K M; Yakovleva, T V; Aldrich, J V et al. (2014) Zyklophin, a short-acting kappa opioid antagonist, induces scratching in mice. Neurosci Lett 563:155-9|
|Lucchesi, Valentina; Hurst, Dow P; Shore, Derek M et al. (2014) CB2-selective cannabinoid receptor ligands: synthesis, pharmacological evaluation, and molecular modeling investigation of 1,8-Naphthyridin-2(1H)-one-3-carboxamides. J Med Chem 57:8777-91|
|Cathel, Alessandra M; Reyes, Beverly A S; Wang, Qin et al. (2014) Cannabinoid modulation of alpha2 adrenergic receptor function in rodent medial prefrontal cortex. Eur J Neurosci 40:3202-14|
Showing the most recent 10 out of 195 publications