The Cell and Immunology Core provides services that allow determination of effects of 1) drugs of abuse, 2) endogenous ligands which bind to the same receptors and transporters used by the drugs of abuse, or 3) infection with HIV or SIV or other opportunistic infections that are common in HIV, on cells of the immune system. The effects that are measured in the Core are functional endpoints which include responses of cells to mitogens;Natural Killer cell activity;capacity for in vitro antibody production;response in the Mixed Lymphocyte Reaction (MLR);and chemotaxis assays. In addition, the Core measures cytokine, chemokine, and neuropeptide levels in serum, plasma, cerebrospinal fluid, bronchial lavage fluid, peritoneal lavage fluid, or in supernatants of cell cultures, by a multiplex assay, or by ELISA assay for individual proteins. In addition, through support of the Flow Cytometry facility, the Core allows determination of effects of treatment with drugs of abuse or other related molecules, or infection, on numbers of immune cells in particular subsets, such as T-cells, CD4 and CD8 T-cells, B-cells, and monocyte/macrophages, Expression of cell surface markers, including receptors on primary cells or transfected cell lines, can also be quantitated on a per cell basis or as the percent of cells expressing a particular molecule. Intracellular expression of cytokines can be determined by flow cytometry. The Core also offers services to separate cells using magnetic bead technology, counter-current centrifugation using an elutriation centrifuge, or through sorting using the flow cytometer. Finally, the Core will screen cell lines for contamination with Mycoplasma.
Exploration of neuroimmune connections is a major theme of the P30 Center. Several projects involve infection with SIV or HIV, combined with drugs of abuse or related molecules. The studies supported by the Core are providing fundamental new knowledge about the interactions of drugs of abuse on the immune system, and how the interactions regulate pain, body temperature, and HIV levels.
|Console-Bram, Linda; Brailoiu, Eugen; Brailoiu, Gabriela Cristina et al. (2014) Activation of GPR18 by cannabinoid compounds: a tale of biased agonism. Br J Pharmacol 171:3908-17|
|Miller, Jonathan S; Barr, Jeffrey L; Harper, Lauren J et al. (2014) The GSK3 signaling pathway is activated by cocaine and is critical for cocaine conditioned reward in mice. PLoS One 9:e88026|
|Zhao, Pingwei; Lane, Tom R; Gao, Helen G L et al. (2014) Crucial positively charged residues for ligand activation of the GPR35 receptor. J Biol Chem 289:3625-38|
|Chatila, W M; Criner, G J; Hancock, W W et al. (2014) Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers. Clin Exp Immunol 177:341-52|
|Enman, Nicole M; Zhang, Yong; Unterwald, Ellen M (2014) Connecting the pathology of posttraumatic stress and substance use disorders: monoamines and neuropeptides. Pharmacol Biochem Behav 117:61-9|
|Shi, Xiangdang; Miller, Jonathan S; Harper, Lauren J et al. (2014) Reactivation of cocaine reward memory engages the Akt/GSK3/mTOR signaling pathway and can be disrupted by GSK3 inhibition. Psychopharmacology (Berl) 231:3109-18|
|Kong, Weimin; Li, Hongbo; Tuma, Ronald F et al. (2014) Selective CB2 receptor activation ameliorates EAE by reducing Th17 differentiation and immune cell accumulation in the CNS. Cell Immunol 287:1-17|
|Dimattio, K M; Yakovleva, T V; Aldrich, J V et al. (2014) Zyklophin, a short-acting kappa opioid antagonist, induces scratching in mice. Neurosci Lett 563:155-9|
|Lucchesi, Valentina; Hurst, Dow P; Shore, Derek M et al. (2014) CB2-selective cannabinoid receptor ligands: synthesis, pharmacological evaluation, and molecular modeling investigation of 1,8-Naphthyridin-2(1H)-one-3-carboxamides. J Med Chem 57:8777-91|
|Cathel, Alessandra M; Reyes, Beverly A S; Wang, Qin et al. (2014) Cannabinoid modulation of alpha2 adrenergic receptor function in rodent medial prefrontal cortex. Eur J Neurosci 40:3202-14|
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