This NIDA P30 Center supplies the resources needed to apply cutting-edge genomic, proteomic, and bioinformatic technologies to the study of chronic viral infections that are a direct consequence of drug abuse and addiction. Over the past four years, our emphasis has been on hepatitis C virus (HCV) infection and its impact on liver function. In this competitive renewal, we propose to augment our work on HCV with an increased emphasis on AIDS and the intravenous drug abuse population. The Center's unifying theme is to integrate the use of global gene expression and protein profiling technologies to develop a detailed understanding of the host response to virus infection and the molecular mechanisms underlying the progression from chronic HCV infection to end-stage liver disease. To achieve this goal, we have brought together a diverse group of NIH-funded investigators, from basic science and clinical medicine, with combined expertise in virology, immunology, liver disease and transplantation, genomics, proteomics, bioinformatics, biostatistics, and technology development;this includes more than 20 key personnel and over 20 other significant contributors from over a dozen academic institutions and three corporations. This remarkable confluence of basic science and clinical medicine, together with genomic, proteomic, and information technologies, represents an unparalleled opportunity to make advances in our understanding HCV-associated liver disease, AIDS, and HCV/HIV-1 dual infection. The Center consists of three scientific Cores and an Administrative Core, which oversees all aspects of the Center. The Functional Genomics &Virology Core provides the biological samples used for our analyses, which include serial liver biopsies from patients with recurrent HCV after liver transplantation, biopsies from patients with HCV-associated liver disease, and liver biopsies from HCV-infected intravenous drug abusers who are undergoing methadone treatment. AIDS-related studies include the analysis of liver biopsies from patients co-infected with HCV and HIV-1 and from a nonhuman primate model of AIDS. The Proteomics Core is located at Pacific Northwest National Laboratory and provides the Center with world-class mass spectrometry instrumentation and state-of-the-art technology development. The Bioinformatics &Biostatistics Core provides the essential functions of data management, analysis, statistical evaluation, and data sharing and dissemination.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
3P30DA015625-10S1
Application #
8479691
Study Section
Special Emphasis Panel (ZDA1-RXL-E (02))
Program Officer
Pollock, Jonathan D
Project Start
2002-09-30
Project End
2013-06-30
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
10
Fiscal Year
2012
Total Cost
$546,235
Indirect Cost
$162,017
Name
University of Washington
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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McDermott, Jason E; Diamond, Deborah L; Corley, Courtney et al. (2012) Topological analysis of protein co-abundance networks identifies novel host targets important for HCV infection and pathogenesis. BMC Syst Biol 6:28

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