The Bioanalytical Core Laboratory will provide state of the art and innovative bioanalytical expertise and techniques for the quantification of drugs and their metabolites, endogenous chemicals and other molecules of biological significance in new collaborative studies with the other cores in the center and all the funded drug abuse biomedical researchers at this and neighboring universities. These analyses will provide the expertise to perform new and innovative research and to enhance currently funded research projects in ways not anticipated at the time of their application submission. This core will accomplish these goals by making available reliable, validated mass spectrometric analysis of biological and non-biological materials for new projects as well as for NIDA sponsored and other researchers studying the mechanism of action of abused substances and addiction. The laboratory will develope methods will focus on the identification and quantification in biologic specimens of drugs and/or drug metabolites, such as cocaine, nicotine, cotinine, tetrahydrocannabinol (THC), JWH-018, JWH-073, CP 47,497, methadrone and methylene, as well as physiologically active small endogenous molecules and/or their metabolites such as anandamide, other endocannabinoids, prostamides and ceramide metabolites of sphingomelingolipids. These analyses will enhance the pharmacological studies of drugs of abuse by providing pharmacokinetic analysis including drug disposition, metabolism and clearance. The Bioanalytical Core will develop novel and innovative techniques for minimum sample preparation to allow rapid isolation and quantification of polar drug metabolites and glucuronide metabolites. If funded, it will develop a research program concerning micro sample preparation techniques such as ambient surface sampling by liquid microjunction surface sampling probe (LMJ-SSP), for MS detection and quantification of drugs or other small molecules in animal tissues and slices. This will allow the detection of drugs/metabolites in specific anatomical areas identified by imaging techniques. We further propose to enhance MSL capabilities to detect analytes at very low concentrations in biological specimens by the addition of micro sampling techniques combined with highly selective and sensitive MS instrumentation such as time of flight (TOF) detectors. Such MS systems decrease the lower limits of detection and improve the quantification of drugs of abuse and their metabolites at very low concentrations in biological specimens. The ability to detect very low drug concentrations will extend the time period for collection specimens for disposition/pharmacokinetic evaluations. The Bioanalytical Core will also collaborate

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
1P30DA033934-01A1
Application #
8577247
Study Section
Special Emphasis Panel (ZDA1-EXL-T (02))
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
1
Fiscal Year
2014
Total Cost
$95,215
Indirect Cost
$32,779
Name
Virginia Commonwealth University
Department
Type
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298
Kang, Minho; Mischel, Ryan A; Bhave, Sukhada et al. (2017) The effect of gut microbiome on tolerance to morphine mediated antinociception in mice. Sci Rep 7:42658
Jacob, Joanna C; Poklis, Justin L; Akbarali, Hamid I et al. (2017) Ethanol Reversal of Tolerance to the Antinociceptive Effects of Oxycodone and Hydrocodone. J Pharmacol Exp Ther 362:45-52
Dempsey, Sara K; Poklis, Justin L; Sweat, Kacie et al. (2017) Acute Toxicity From Intravenous Use of the Tricyclic Antidepressant Tianeptine. J Anal Toxicol 41:547-550
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Li, Guangbi; Chen, Zhida; Bhat, Owais M et al. (2017) NLRP3 inflammasome as a novel target for docosahexaenoic acid metabolites to abrogate glomerular injury. J Lipid Res 58:1080-1090
Wolf, Carl E; Poklis, Justin L; Cumpston, Kirk et al. (2017) Acute dilated cardiomyopathy and myocardial injury after combined 4-fluoroamphetamine and modafinil ingestion. Drug Test Anal 9:657-659
Shin, Myungsun; Snyder, Helena W; Donvito, Giulia et al. (2017) Liposomal Delivery of Diacylglycerol Lipase-Beta Inhibitors to Macrophages Dramatically Enhances Selectivity and Efficacy in Vivo. Mol Pharm :
Wolf, Carl E; Poklis, Justin L; Poklis, Alphonse (2017) Stability of Tetrahydrocannabinol and Cannabidiol in Prepared Quality Control Medible Brownies. J Anal Toxicol 41:153-157
Poklis, Justin L; Wolf 2nd, Carl E; Peace, Michelle R (2017) Ethanol concentration in 56 refillable electronic cigarettes liquid formulations determined by headspace gas chromatography with flame ionization detector (HS-GC-FID). Drug Test Anal 9:1637-1640
Akbarali, Hamid I; Dewey, William L (2017) The gut-brain interaction in opioid tolerance. Curr Opin Pharmacol 37:126-130

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