The BAC Recombineering and Transgenic Targeting Core (BRTT) will generate, characterize and distribute specialized transgenic lines requested by the NIDA PSO Center for Molecular and Epigenetic Research of Cell Types Mediating Addictive Behaviors. The BRTT core will be in charge of the following subprojects/tasks: 1) BAC modification of Cre-recombinase and TRAP vectors, 2) Generation of BAC transgenic mice, 3) Histological confirmation of accurate transgene expression, and 4) Archive and distribution of BAC transgenic mice

Public Health Relevance

There is great need for cell-type specific transgenic mouse lines that genetically label neuronal populations involved in addictive behaviors to be employed for high throughput translational profiling and to foster a better understanding of the molecular processes underlying drug addiction and chronic pain disorders and accelerate drug development

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Center Core Grants (P30)
Project #
1P30DA035756-01
Application #
8577653
Study Section
Special Emphasis Panel (ZDA1-EXL-T (02))
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-02-28
Support Year
1
Fiscal Year
2013
Total Cost
$420,501
Indirect Cost
$172,418
Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Broms, Jonas; Antolin-Fontes, Beatriz; Tingström, Anders et al. (2015) Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates. J Comp Neurol 523:359-80
Fishell, Gord; Heintz, Nathaniel (2013) The neuron identity problem: form meets function. Neuron 80:602-12