The BAC Recombineering and Transgenic Targeting Core (BRTT) will generate, characterize and distribute specialized transgenic lines requested by the NIDA PSO Center for Molecular and Epigenetic Research of Cell Types Mediating Addictive Behaviors. The BRTT core will be in charge of the following subprojects/tasks: 1) BAC modification of Cre-recombinase and TRAP vectors, 2) Generation of BAC transgenic mice, 3) Histological confirmation of accurate transgene expression, and 4) Archive and distribution of BAC transgenic mice

Public Health Relevance

There is great need for cell-type specific transgenic mouse lines that genetically label neuronal populations involved in addictive behaviors to be employed for high throughput translational profiling and to foster a better understanding of the molecular processes underlying drug addiction and chronic pain disorders and accelerate drug development.

National Institute of Health (NIH)
National Institute on Drug Abuse (NIDA)
Center Core Grants (P30)
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Special Emphasis Panel (ZDA1-EXL-T)
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Rockefeller University
New York
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Nakayama, Hirofumi; Ibañez-Tallon, Ines; Heintz, Nathaniel (2018) Cell-Type-Specific Contributions of Medial Prefrontal Neurons to Flexible Behaviors. J Neurosci 38:4490-4504
Nectow, Alexander R; Moya, Maria V; Ekstrand, Mats I et al. (2017) Rapid Molecular Profiling of Defined Cell Types Using Viral TRAP. Cell Rep 19:655-667
Abraira, Victoria E; Kuehn, Emily D; Chirila, Anda M et al. (2017) The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn. Cell 168:295-310.e19
Ables, Jessica L; Görlich, Andreas; Antolin-Fontes, Beatriz et al. (2017) Retrograde inhibition by a specific subset of interpeduncular ?5 nicotinic neurons regulates nicotine preference. Proc Natl Acad Sci U S A 114:13012-13017
Li, Kun; Nakajima, Miho; Ibañez-Tallon, Ines et al. (2016) A Cortical Circuit for Sexually Dimorphic Oxytocin-Dependent Anxiety Behaviors. Cell 167:60-72.e11
Baddack, Uta; Frahm, Silke; Antolin-Fontes, Beatriz et al. (2015) Suppression of Peripheral Pain by Blockade of Voltage-Gated Calcium 2.2 Channels in Nociceptors Induces RANKL and Impairs Recovery From Inflammatory Arthritis in a Mouse Model. Arthritis Rheumatol 67:1657-67
Frahm, Silke; Antolin-Fontes, Beatriz; Görlich, Andreas et al. (2015) An essential role of acetylcholine-glutamate synergy at habenular synapses in nicotine dependence. Elife 4:e11396
Antolin-Fontes, Beatriz; Ables, Jessica L; Görlich, Andreas et al. (2015) The habenulo-interpeduncular pathway in nicotine aversion and withdrawal. Neuropharmacology 96:213-22
Shrestha, Prerana; Mousa, Awni; Heintz, Nathaniel (2015) Layer 2/3 pyramidal cells in the medial prefrontal cortex moderate stress induced depressive behaviors. Elife 4:
Broms, Jonas; Antolin-Fontes, Beatriz; Tingström, Anders et al. (2015) Conserved expression of the GPR151 receptor in habenular axonal projections of vertebrates. J Comp Neurol 523:359-80

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