Over ttie past 2 decades, advances in mouse molecular genetics tiave lead to significant discoveries in neural function and development, findings that simply are not attainable without these valuable animal resources. The widespread use of genetically engineered mice has resulted in a huge array of available altered strains, which are shared widely among the research community. Additionally, the technology for generating genetically altered mice has continued to improve, and has become a conventional tool for manipulation of candidate genes. Importantly, conditional mouse genetic lines are now readily available that allow temporal and tissue specific manipulation of genes, either transiently, or indelibly. Researchers in the RMTSC employ mouse lines representing each of these aspects of cutting edge mouse molecular genetics, and in doing so, are advancing the field significantly. The primary function of Core A is to support RMTSC researcher use of mice, by maintaining breeding stocks of genetically altered mice, assisting in developing complex breeding strategies to produce offspring with the correct engineered alleles for genetic experiments, providing highly efficient genotyping of offspring, and imparting concise technical advice in the design of constructs for the genesis of new mouse lines. Given the success of our Core A in supporting mouse work through these services to RMTSC researchers in the past 9 years, the 3 overarching aims of this Core proposal remain unchanged from those ofthe previous submission.
Aim 1. To maintain genetically altered mice.
Aim 2. To genotype offspring of genetically altered mice..
Aim 3. To advise investigators in designing constructs for generation of genetically altered mice.

National Institute of Health (NIH)
National Institute on Deafness and Other Communication Disorders (NIDCD)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDC1-SRB-Q)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Colorado Denver
United States
Zip Code
Hansen, Anne; Ghosal, Ratna; Caprio, John et al. (2014) Anatomical and physiological studies of bigheaded carps demonstrate that the epibranchial organ functions as a pharyngeal taste organ. J Exp Biol 217:3945-54
Ganchrow, Donald; Ganchrow, Judith R; Cicchini, Vanessa et al. (2014) Nucleus of the solitary tract in the C57BL/6J mouse: Subnuclear parcellation, chorda tympani nerve projections, and brainstem connections. J Comp Neurol 522:1565-96
Nunez-Parra, Alexia; Li, Anan; Restrepo, Diego (2014) Coding odor identity and odor value in awake rodents. Prog Brain Res 208:205-22
Rorabaugh, Jacki M; Stratford, Jennifer M; Zahniser, Nancy R (2014) A relationship between reduced nucleus accumbens shell and enhanced lateral hypothalamic orexin neuronal activation in long-term fructose bingeing behavior. PLoS One 9:e95019
Sclafani, Anthony; Ackroff, Karen (2014) Maltodextrin and fat preference deficits in "taste-blind" P2X2/P2X3 knockout mice. Chem Senses 39:507-14
Castillo, David; Seidel, Kerstin; Salcedo, Ernesto et al. (2014) Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium. Development 141:2993-3002
Saunders, Cecil J; Christensen, Michael; Finger, Thomas E et al. (2014) Cholinergic neurotransmission links solitary chemosensory cells to nasal inflammation. Proc Natl Acad Sci U S A 111:6075-80
Stratford, Jennifer M; Thompson, John A (2014) Beta-galactosidase staining in the nucleus of the solitary tract of Fos-Tau-LacZ mice is unaffected by monosodium glutamate taste stimulation. PLoS One 9:e107238
Frenz, Christopher T; Hansen, Anne; Dupuis, Nicholas D et al. (2014) NaV1.5 sodium channel window currents contribute to spontaneous firing in olfactory sensory neurons. J Neurophysiol 112:1091-104
Sheridan, D C; Hughes, A R; Erdélyi, F et al. (2014) Matching of feedback inhibition with excitation ensures fidelity of information flow in the anterior piriform cortex. Neuroscience 275:519-30

Showing the most recent 10 out of 106 publications