Over ttie past 2 decades, advances in mouse molecular genetics tiave lead to significant discoveries in neural function and development, findings that simply are not attainable without these valuable animal resources. The widespread use of genetically engineered mice has resulted in a huge array of available altered strains, which are shared widely among the research community. Additionally, the technology for generating genetically altered mice has continued to improve, and has become a conventional tool for manipulation of candidate genes. Importantly, conditional mouse genetic lines are now readily available that allow temporal and tissue specific manipulation of genes, either transiently, or indelibly. Researchers in the RMTSC employ mouse lines representing each of these aspects of cutting edge mouse molecular genetics, and in doing so, are advancing the field significantly. The primary function of Core A is to support RMTSC researcher use of mice, by maintaining breeding stocks of genetically altered mice, assisting in developing complex breeding strategies to produce offspring with the correct engineered alleles for genetic experiments, providing highly efficient genotyping of offspring, and imparting concise technical advice in the design of constructs for the genesis of new mouse lines. Given the success of our Core A in supporting mouse work through these services to RMTSC researchers in the past 9 years, the 3 overarching aims of this Core proposal remain unchanged from those ofthe previous submission.
Aim 1. To maintain genetically altered mice.
Aim 2. To genotype offspring of genetically altered mice..
Aim 3. To advise investigators in designing constructs for generation of genetically altered mice.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30DC004657-15
Application #
8725114
Study Section
Special Emphasis Panel (ZDC1)
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Type
DUNS #
City
Aurora
State
CO
Country
United States
Zip Code
80045
von Holstein-Rathlou, Stephanie; BonDurant, Lucas D; Peltekian, Lila et al. (2016) FGF21 Mediates Endocrine Control of Simple Sugar Intake and Sweet Taste Preference by the Liver. Cell Metab 23:335-43
Ye, Wenlei; Chang, Rui B; Bushman, Jeremy D et al. (2016) The K+ channel KIR2.1 functions in tandem with proton influx to mediate sour taste transduction. Proc Natl Acad Sci U S A 113:E229-38
Stratford, Jennifer M; Thompson, John A (2016) MSG-Evoked c-Fos Activity in the Nucleus of the Solitary Tract Is Dependent upon Fluid Delivery and Stimulation Parameters. Chem Senses 41:211-20
Chang, Weipang; Kanda, Hirosato; Ikeda, Ryo et al. (2016) Merkel disc is a serotonergic synapse in the epidermis for transmitting tactile signals in mammals. Proc Natl Acad Sci U S A 113:E5491-500
Larson, Eric D; Vandenbeuch, Aurelie; Voigt, Anja et al. (2015) The Role of 5-HT3 Receptors in Signaling from Taste Buds to Nerves. J Neurosci 35:15984-95
Vandenbeuch, Aurelie; Larson, Eric D; Anderson, Catherine B et al. (2015) Postsynaptic P2X3-containing receptors in gustatory nerve fibres mediate responses to all taste qualities in mice. J Physiol 593:1113-25
Gaillard, Dany; Xu, Mingang; Liu, Fei et al. (2015) β-Catenin Signaling Biases Multipotent Lingual Epithelial Progenitors to Differentiate and Acquire Specific Taste Cell Fates. PLoS Genet 11:e1005208
Ozbay, Baris N; Losacco, Justin T; Cormack, Robert et al. (2015) Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts. Opt Lett 40:2553-6
Vandenbeuch, Aurelie; Anderson, Catherine B; Kinnamon, Sue C (2015) Mice Lacking Pannexin 1 Release ATP and Respond Normally to All Taste Qualities. Chem Senses 40:461-7
Rorabaugh, J M; Stratford, J M; Zahniser, N R (2015) Differences in bingeing behavior and cocaine reward following intermittent access to sucrose, glucose or fructose solutions. Neuroscience 301:213-20

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