This competing renewal for the RMTSC Core on Cellular Visualization and Analysis seeks to continue support services for histology, neuroanatomy and advanced microscopy for RMTSC investigators. This Core offers 3 types of services: 1) training and expertise in specialized histology (e.g. immunocytochemistry) and neuroanatomy, 2) Specialized microscopy facilities and training, including confocal microscopy and digital imaging, and 3) Quantitative analysis and representation of 3D anatomical data. This Core has been highly successful in the past grant period, being involved in experiments from all RMTSC participating laboratories and assisting in most publications arising from the Center. We propose to continue these functions into the next grant period with only minor modifications to the approach and services offered. The principal functions of this Core include provision of training and assistance in the development and application of contemporary histological methods with an emphasis on immunocytochemistry and in situ hybridization. The Core offers quality control and advice in proper utilization of immunological reagents. In addition, the Core provides anatomical expertise to enable investigators from other fields to properly analyze and interpret anatomical images. In terms of microscopy equipment, the Core maintains a RMTSC laser scanning confocal microscope and supports use of other advanced microscopes including spinning disk confocal, 2-photon confocal, Ca-lmaging workstations and transmission electron microscopes. Finally, the Cellular Visualization and Analysis Core offers expertise and development of software capable of rendering 3-D anatomical data in a quantitative enabled format to permit objective measures of changes in activity patterns, endorgan distribution and cellular activity.
Imaging technology has advanced in the last decades to permit correlative anatomical/functional analyses of biomedical samples ranging from the system to sub-cellular and molecular levels. This Core facilitates utilization of these methodologies by RMTSC participants investigating the function of chemosensory systems in health and in disease states.
|von Holstein-Rathlou, Stephanie; BonDurant, Lucas D; Peltekian, Lila et al. (2016) FGF21 Mediates Endocrine Control of Simple Sugar Intake and Sweet Taste Preference by the Liver. Cell Metab 23:335-43|
|Ye, Wenlei; Chang, Rui B; Bushman, Jeremy D et al. (2016) The K+ channel KIR2.1 functions in tandem with proton influx to mediate sour taste transduction. Proc Natl Acad Sci U S A 113:E229-38|
|Stratford, Jennifer M; Thompson, John A (2016) MSG-Evoked c-Fos Activity in the Nucleus of the Solitary Tract Is Dependent upon Fluid Delivery and Stimulation Parameters. Chem Senses 41:211-20|
|Chang, Weipang; Kanda, Hirosato; Ikeda, Ryo et al. (2016) Merkel disc is a serotonergic synapse in the epidermis for transmitting tactile signals in mammals. Proc Natl Acad Sci U S A 113:E5491-500|
|Larson, Eric D; Vandenbeuch, Aurelie; Voigt, Anja et al. (2015) The Role of 5-HT3 Receptors in Signaling from Taste Buds to Nerves. J Neurosci 35:15984-95|
|Vandenbeuch, Aurelie; Larson, Eric D; Anderson, Catherine B et al. (2015) Postsynaptic P2X3-containing receptors in gustatory nerve fibres mediate responses to all taste qualities in mice. J Physiol 593:1113-25|
|Gaillard, Dany; Xu, Mingang; Liu, Fei et al. (2015) Î²-Catenin Signaling Biases Multipotent Lingual Epithelial Progenitors to Differentiate and Acquire Specific Taste Cell Fates. PLoS Genet 11:e1005208|
|Ozbay, Baris N; Losacco, Justin T; Cormack, Robert et al. (2015) Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts. Opt Lett 40:2553-6|
|Vandenbeuch, Aurelie; Anderson, Catherine B; Kinnamon, Sue C (2015) Mice Lacking Pannexin 1 Release ATP and Respond Normally to All Taste Qualities. Chem Senses 40:461-7|
|Rorabaugh, J M; Stratford, J M; Zahniser, N R (2015) Differences in bingeing behavior and cocaine reward following intermittent access to sucrose, glucose or fructose solutions. Neuroscience 301:213-20|
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