This competing renewal for the RMTSC Core on Cellular Visualization and Analysis seeks to continue support services for histology, neuroanatomy and advanced microscopy for RMTSC investigators. This Core offers 3 types of services: 1) training and expertise in specialized histology (e.g. immunocytochemistry) and neuroanatomy, 2) Specialized microscopy facilities and training, including confocal microscopy and digital imaging, and 3) Quantitative analysis and representation of 3D anatomical data. This Core has been highly successful in the past grant period, being involved in experiments from all RMTSC participating laboratories and assisting in most publications arising from the Center. We propose to continue these functions into the next grant period with only minor modifications to the approach and services offered. The principal functions of this Core include provision of training and assistance in the development and application of contemporary histological methods with an emphasis on immunocytochemistry and in situ hybridization. The Core offers quality control and advice in proper utilization of immunological reagents. In addition, the Core provides anatomical expertise to enable investigators from other fields to properly analyze and interpret anatomical images. In terms of microscopy equipment, the Core maintains a RMTSC laser scanning confocal microscope and supports use of other advanced microscopes including spinning disk confocal, 2-photon confocal, Ca-lmaging workstations and transmission electron microscopes. Finally, the Cellular Visualization and Analysis Core offers expertise and development of software capable of rendering 3-D anatomical data in a quantitative enabled format to permit objective measures of changes in activity patterns, endorgan distribution and cellular activity.
Imaging technology has advanced in the last decades to permit correlative anatomical/functional analyses of biomedical samples ranging from the system to sub-cellular and molecular levels. This Core facilitates utilization of these methodologies by RMTSC participants investigating the function of chemosensory systems in health and in disease states.
|Hansen, Anne; Ghosal, Ratna; Caprio, John et al. (2014) Anatomical and physiological studies of bigheaded carps demonstrate that the epibranchial organ functions as a pharyngeal taste organ. J Exp Biol 217:3945-54|
|Ganchrow, Donald; Ganchrow, Judith R; Cicchini, Vanessa et al. (2014) Nucleus of the solitary tract in the C57BL/6J mouse: Subnuclear parcellation, chorda tympani nerve projections, and brainstem connections. J Comp Neurol 522:1565-96|
|Nunez-Parra, Alexia; Li, Anan; Restrepo, Diego (2014) Coding odor identity and odor value in awake rodents. Prog Brain Res 208:205-22|
|Rorabaugh, Jacki M; Stratford, Jennifer M; Zahniser, Nancy R (2014) A relationship between reduced nucleus accumbens shell and enhanced lateral hypothalamic orexin neuronal activation in long-term fructose bingeing behavior. PLoS One 9:e95019|
|Sclafani, Anthony; Ackroff, Karen (2014) Maltodextrin and fat preference deficits in "taste-blind" P2X2/P2X3 knockout mice. Chem Senses 39:507-14|
|Castillo, David; Seidel, Kerstin; Salcedo, Ernesto et al. (2014) Induction of ectopic taste buds by SHH reveals the competency and plasticity of adult lingual epithelium. Development 141:2993-3002|
|Saunders, Cecil J; Christensen, Michael; Finger, Thomas E et al. (2014) Cholinergic neurotransmission links solitary chemosensory cells to nasal inflammation. Proc Natl Acad Sci U S A 111:6075-80|
|Stratford, Jennifer M; Thompson, John A (2014) Beta-galactosidase staining in the nucleus of the solitary tract of Fos-Tau-LacZ mice is unaffected by monosodium glutamate taste stimulation. PLoS One 9:e107238|
|Frenz, Christopher T; Hansen, Anne; Dupuis, Nicholas D et al. (2014) NaV1.5 sodium channel window currents contribute to spontaneous firing in olfactory sensory neurons. J Neurophysiol 112:1091-104|
|Sheridan, D C; Hughes, A R; Erdélyi, F et al. (2014) Matching of feedback inhibition with excitation ensures fidelity of information flow in the anterior piriform cortex. Neuroscience 275:519-30|
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