The sensory organs of the inner ear are among the most complex and exquisitely-patterned structures contained within the vertebrate body. Research on the biological mechanisms of hearing and balance requires the ability to obtain high-resolution images of the cochlea and vestibular organs, as well as their associated tissues. Modern optical and electron microscopy can provide excellent imaging data from processed samples and live tissues, but the cost of such instrumentation is typically beyond the reach of individual labs. The most cost-effective method for providing advanced microscopy services to a number of labs is for those labs to form a consortium and share imaging facilities. The present proposal is for the continuation of a P30 project that is directed toward this goal. Specifically, the Molecular and Digital Imaging (MDI) core will provide access to modern optical and electron microscopy to a group of associated labs whose research is consistent with the mission aims of the NIDCD. This section of the proposal has two Specific Aims: First, we propose to provide facilities for optical microscopy (confocal and conventional epifluorescence) to our core group of researchers, along with complete training in the operation of those instruments. The second Specific Aim is to provide access to both scanning and transmission electron microscopy services to this same group of researchers, along with equipment for sample preparation and training in all facets of EM technique. Both optical and EM imaging are heavily reliant on digital image processing, so we will also provide complete computational resources to users of our shared facility. Our core research group is large and diverse, but we are united by many common goals. In addition, we foster numerous collaborative projects, many of which have been initiated and facilitated by the MDI core resources. The MDI core has established an excellent track record of utilization and research productivity. During the previous funding cycle, we have provided imaging instrumentation and training to a total of 37 labs, and data obtained from MDI core facilities has appeared in 56 peer-reviewed publications. In light of this high level of productivity, along with our newly upgraded confocal facility and strong research group, we seek to continue to promote the research of our member labs, and increase existing knowledge of the biological basis of hearing and balance and associated pathologies.
The P30 Research Core Center provides access to instrumentation, technical services and training, in order to facilitate the scientific progress of investigators conducting research in mission areas of the NIDCD. We provide shared resources that would otherwise be unavailable to individual labs, and also promote scientific interaction, collaboration and translational research. Highlighted areas of support include Functional Testing, Histology, Microscopy &Digital Imaging, and Clinical &Translational Research.
|Rakszawski, Bernadette; Wright, Rose; Cadieux, Jamie H et al. (2016) The Effects of Preprocessing Strategies for Pediatric Cochlear Implant Recipients. J Am Acad Audiol 27:85-102|
|Meehan, Daniel T; Delimont, Duane; Dufek, Brianna et al. (2016) Endothelin-1 mediated induction of extracellular matrix genes in strial marginal cells underlies strial pathology in Alport mice. Hear Res 341:100-108|
|Mulay, Shrikant R; Desai, Jyaysi; Kumar, Santhosh V et al. (2016) Cytotoxicity of crystals involves RIPK3-MLKL-mediated necroptosis. Nat Commun 7:10274|
|Dufek, Brianna; Meehan, Daniel T; Delimont, Duane et al. (2016) Endothelin A receptor activation on mesangial cellsÂ initiates Alport glomerular disease. Kidney Int 90:300-10|
|Kaur, Tejbeer; Zamani, Darius; Tong, Ling et al. (2015) Fractalkine Signaling Regulates Macrophage Recruitment into the Cochlea and Promotes the Survival of Spiral Ganglion Neurons after Selective Hair Cell Lesion. J Neurosci 35:15050-61|
|Huh, Sung-Ho; Warchol, Mark E; Ornitz, David M (2015) Cochlear progenitor number is controlled through mesenchymal FGF receptor signaling. Elife 4:|
|Gehlhausen, Jeffrey R; Park, Su-Jung; Hickox, Ann E et al. (2015) A murine model of neurofibromatosis type 2 that accurately phenocopies human schwannoma formation. Hum Mol Genet 24:1-8|
|Chen, Shuang; Brunskill, Eric W; Potter, S Steven et al. (2015) Intrinsic Age-Dependent Changes and Cell-Cell Contacts Regulate Nephron Progenitor Lifespan. Dev Cell 35:49-62|
|de la Puente, Pilar; Muz, Barbara; Gilson, Rebecca C et al. (2015) 3D tissue-engineered bone marrow as a novel model to study pathophysiology and drug resistance in multiple myeloma. Biomaterials 73:70-84|
|Gasser Rutledge, Krysta L; Prasad, Kumar G; Emery, Kara R et al. (2015) Short-term Peripheral Auditory Effects of Cranial Irradiation: A Mouse Model. Ann Otol Rhinol Laryngol 124:903-10|
Showing the most recent 10 out of 142 publications