Inner and middle ear tissues are among the most difficult to properly fix, orient and section for routine light and electron microscopy. However, this approach is crucial to obtain a proper understanding of the development and aging, function, disease processes and recovery of the inner and middle ear. The goal of the Histology Core is to provide the necessary training and/or services that will enable any investigator to routinely examine inner ear and related structures. Tissues of interest include, but are not limited to the sensory epithelium, bone and nervous system. The core provides full service histology, training and equipment use for frozen tissues or tissues embedded in paraffin as well as methacrylate and epoxy resins. Thin sectioning for electron microscopy is also available as are a wide variety of counterstaining and enzyme staining procedures.
The specific aims of the Histology Core are: 1) to ensure that member investigators utilize optimal histological processing of desired tissues through consultation, training, supervision and evaluation of results as well as through the provision of technical services;2) to promote collaborative efforts between core members as well as outside investigators;and 3) to promote the rapid systematic evaluation of novel research models including new knockout and transgenic mice as well as innovative experimental paradigms. The histology core interacts intimately with other cores. Collaborative efforts with the Functional Testing and Molecular &Digital Imaging Cores provide for a unique ability to assess inner/middle ear function and anatomy in a quick and comparative fashion. This broad spectrum of services provides an appealing environment that encourages collaborative interactions both within the core membership and with investigators from other fields. Anatomy-based research is becoming a lost art with the current emphasis on molecular techniques and many labs can no longer afford the specialized equipment and personnel required to optimally perform these procedures. The Histology Core provides a vital service to ensure that this approach is always available for investigators in our field.

Public Health Relevance

The P30 Research Core Center creates centralized resources to facilitate the scientific progress of investigators conducting research in mission areas of the NIDCD. It provides technical assistance, training, and equipment at a level that is not feasible for individual investigators and is designed to promote scientific interaction, collaboration and translational research. Highlighted areas of support include Functional Testing, Histology, Microscopy &Digital Imaging, and Clinical &Translational Research.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30DC004665-14
Application #
8725633
Study Section
Special Emphasis Panel (ZDC1)
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Washington University
Department
Type
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
Slattery, Eric L; Oshima, Kazuo; Heller, Stefan et al. (2014) Cisplatin exposure damages resident stem cells of the mammalian inner ear. Dev Dyn 243:1328-37
Chole, Richard A; Gagnon, Patricia M; Vogel, Joseph P (2014) Inactivation of specific Pseudomonas aeruginosa biofilm factors does not alter virulence in infected cholesteatomas. Otol Neurotol 35:1585-91
Kim, Sung Tae; Ahn, Sun-Young; Swat, Wojciech et al. (2014) DLG1 influences distal ureter maturation via a non-epithelial cell autonomous mechanism involving reduced retinoic acid signaling, Ret expression, and apoptosis. Dev Biol 390:160-9
Onken, Michael D; Winkler, Ashley E; Kanchi, Krishna-Latha et al. (2014) A surprising cross-species conservation in the genomic landscape of mouse and human oral cancer identifies a transcriptional signature predicting metastatic disease. Clin Cancer Res 20:2873-84
Tran, Nicholas M; Zhang, Alan; Zhang, Xiaodong et al. (2014) Mechanistically distinct mouse models for CRX-associated retinopathy. PLoS Genet 10:e1004111
Ku, Yuan-Chieh; Renaud, Nicole A; Veile, Rose A et al. (2014) The transcriptome of utricle hair cell regeneration in the avian inner ear. J Neurosci 34:3523-35
Dwyer, Noel Y; Firszt, Jill B; Reeder, Ruth M (2014) Effects of unilateral input and mode of hearing in the better ear: self-reported performance using the speech, spatial and qualities of hearing scale. Ear Hear 35:126-36
Sharon, Jeffrey D; Khwaja, Shariq S; Drescher, Andrew et al. (2014) Osteoradionecrosis of the temporal bone: a case series. Otol Neurotol 35:1207-17
Sinha, P; Thorstad, W T; Nussenbaum, B et al. (2014) Distant metastasis in p16-positive oropharyngeal squamous cell carcinoma: a critical analysis of patterns and outcomes. Oral Oncol 50:45-51
Huh, Sung-Ho; Narhi, Katja; Lindfors, Paivi H et al. (2013) Fgf20 governs formation of primary and secondary dermal condensations in developing hair follicles. Genes Dev 27:450-8

Showing the most recent 10 out of 103 publications