The Histology and Imaging core supports analysis of mutant ears and connectional defects at all levels in fixed tissue. The goal of this core is to provide the necessary training and certain services to process and image ears at all light and electron microscopic levels, including high resolution multiphoton imaging using multicolor labeling and 3D reconstruction to accelerate analysis of existing and soon to be analyzed mutants or otherwise manipulated ears, including samples of human ears as needed. To this end, the core provides expertise through the core personnel, including a full time RA expertly trained in all techniques needed to evaluate ears and neuronal connections at all stages of development. Reservation of the equipment in this core (three confocal systems, high resolution transmitted and epifluorescence microscopes and dissecting scopes, TEM and SEM, freezing microtomes, vibratomes and ultramicrotomes) will be handled on line.
The specific aims of the histology and imaging core are: (1) To provide a range of service (advice and/or assistance) for optimized and standardized histological processing at all levels of light and electron microscopy, including sophisticated multicolor immunocytochemical labeling followed by multiphoton analysis, or combinations of in situ hybridization and immunocytochemistry;(2) To improve data analysis through the availability and use of appropriate image processing and quantitative analysis software;(3) To provide expert training in these techniques to stimulate collaboration between members of the core grant as well as potential additional members currently funded through NIDCD. The histology core closely interacts with other cores such as the molecular core for the generation of in situ probes. The core will be driven by the needs of several researchers to achieve a standardized high resolution analysis of various mouse models generated by genetic or other manipulations. The scientific expertise of the core personnel will enhance the performance of all core related research. An annual workshop that provides overviews of the techniques available in this core and their potential usefulness for research of current and future members of the core will generate a vital interaction toward a hearing research related community at the Univ. of Iowa.

Public Health Relevance

The histology and imaging core provides centralized resources and facilities that are readily available to all NIDCD funded investigators at the University of Iowa (currently 19 R01 funded investigators) as well as to other NIH funded investigators at this University. The availability of this core will enhance ongoing NIDCD funded research.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Center Core Grants (P30)
Project #
5P30DC010362-05
Application #
8721916
Study Section
Special Emphasis Panel (ZDC1)
Project Start
Project End
Budget Start
2014-09-01
Budget End
2015-08-31
Support Year
5
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Iowa
Department
Type
DUNS #
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Kay, Alan R; Raccuglia, Davide; Scholte, Jon et al. (2016) Goggatomy: A Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments. Front Physiol 7:398
Eyo, Ukpong B; Miner, Samuel A; Weiner, Joshua A et al. (2016) Developmental changes in microglial mobilization are independent of apoptosis in the neonatal mouse hippocampus. Brain Behav Immun 55:49-59
Saito, Yuhki; Miranda-Rottmann, Soledad; Ruggiu, Matteo et al. (2016) NOVA2-mediated RNA regulation is required for axonal pathfinding during development. Elife 5:
Fritzsch, Bernd; Duncan, Jeremy S; Kersigo, Jennifer et al. (2016) Neuroanatomical Tracing Techniques in the Ear: History, State of the Art, and Future Developments. Methods Mol Biol 1427:243-62
Li, Tongchao; Giagtzoglou, Nikolaos; Eberl, Daniel F et al. (2016) The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals. Elife 5:
Yang, Tian; Scholl, Elizabeth S; Pan, Ning et al. (2016) Expression and Localization of CaBP Ca2+ Binding Proteins in the Mouse Cochlea. PLoS One 11:e0147495
Elliott, Karen L; Houston, Douglas W; DeCook, Rhonda et al. (2015) Ear manipulations reveal a critical period for survival and dendritic development at the single-cell level in Mauthner neurons. Dev Neurobiol 75:1339-51
Karak, Somdatta; Jacobs, Julie S; Kittelmann, Maike et al. (2015) Diverse Roles of Axonemal Dyneins in Drosophila Auditory Neuron Function and Mechanical Amplification in Hearing. Sci Rep 5:17085
Ahmad, Iram; Fernando, Augusta; Gurgel, Richard et al. (2015) Merlin status regulates p75(NTR) expression and apoptotic signaling in Schwann cells following nerve injury. Neurobiol Dis 82:114-22
Kopelovich, Jonathan C; Reiss, Lina A J; Etler, Christine P et al. (2015) Hearing Loss After Activation of Hearing Preservation Cochlear Implants Might Be Related to Afferent Cochlear Innervation Injury. Otol Neurotol 36:1035-44

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