The imaging core has four independent workstafions (Image Stafions 1-4), each incorporafing overlapping but independent imaging technologies. Three of four can be used for live cell imaging and two of four will have complementary electrophysiological recording stafions that allow the combinafion of powerful imaging and electrophysiological technologies. From an imaging perspective, the core facilities can accommodate multi-fluorophore immunocytochemical high-resolufion 3-dimensional imaging while also serving high speed physiological imaging. Addifionally, there are two dedicated computers with high end graphics boards, ample memory and processing power that can be used for image analysis offline from the imaging stafions. From conventional fluorescence and confocal imaging to high speed swept field confocal and two-photon imaging and uncaging, the core facility will serve to bring together ROI-funded invesfigators with a broad range of interests and expertise. The core will facilitate the use of this equipment so that translafional uses by the clinical faculty can be increased while at the same fime having the versafility to include users with common interests from mulfiple departments that share goals that are in agreement with the mission statement of the NIDCD. Clear examples of each of these uses exist already with the two-photon system being used to develop whole animal cochlea imaging that hopes to be ufilized in cochlear implant placements, while both the high speed swept field confocal system and the two photon system are presently being used to invesfigate synchronous firing in the cortex. This core grant proposal will allow us to expand the number of users and thus serve as a means to increase collaborafion across both basic science and clinical departments

National Institute of Health (NIH)
National Institute on Deafness and Other Communication Disorders (NIDCD)
Center Core Grants (P30)
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Special Emphasis Panel (ZDC1-SRB-Q)
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Stanford University
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Cox, Brandon C; Chai, Renjie; Lenoir, Anne et al. (2014) Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development 141:816-29
Calton, Melissa A; Lee, Dasom; Sundaresan, Srividya et al. (2014) A lack of immune system genes causes loss in high frequency hearing but does not disrupt cochlear synapse maturation in mice. PLoS One 9:e94549
Ronaghi, Mohammad; Nasr, Marjan; Ealy, Megan et al. (2014) Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev 23:1275-84
Gao, Simon S; Wang, Rosalie; Raphael, Patrick D et al. (2014) Vibration of the organ of Corti within the cochlear apex in mice. J Neurophysiol 112:1192-204
Mendus, Diana; Sundaresan, Srividya; Grillet, Nicolas et al. (2014) Thrombospondins 1 and 2 are important for afferent synapse formation and function in the inner ear. Eur J Neurosci 39:1256-67
Durruthy-Durruthy, Robert; Gottlieb, Assaf; Hartman, Byron H et al. (2014) Reconstruction of the mouse otocyst and early neuroblast lineage at single-cell resolution. Cell 157:964-78
Aguilar, Andrea; Becker, Lars; Tedeschi, Thomas et al. (2014) ?-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion. Mol Biol Cell 25:1854-66
Guo, Zhaohua; Grimm, Christian; Becker, Lars et al. (2013) A novel ion channel formed by interaction of TRPML3 with TRPV5. PLoS One 8:e58174
Volkenstein, Stefan; Oshima, Kazuo; Sinkkonen, Saku T et al. (2013) Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus. Proc Natl Acad Sci U S A 110:14456-61
Cao, Huiren; Yin, Xiaolei; Cao, Yujie et al. (2013) FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PLoS One 8:e56516

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