Existing and planned experimental work in several NIDCD ROI-funded laboratories requires sophlsficated measures of auditory function in small mammals. The breadth of these auditory funcfion measures ranges from simple high-throughput screening up to full comprehensive, frequency-specific measures concerning the middle ear, outer hair cell funcfion, and neural funcfion. These measures can be tailored for simple screening to frequency-specific threshold estimates. Measures of middle ear function will be added. The Core Auditory Function facility will ensure that these complex auditory measures are performed efficiently and accurately with the appropriate apparatus, under standardized, and calibrated condifions, and managed with a comprehensive database that stores, manages and retrieves individual and aggregate experimental auditory data. The Auditory Function Core consists of a Central Auditory Laboratory, three individual auditory measurement facilities, and a noise damaging facility, all in close proximity. The facilifies have convenfional apparatus and many custom components including high-frequency transducers for specific species. Roufine measures include distortion product otoacousfic emissions and frequency-specific auditory measures at the level ofthe brainstem tailored to the specific small mammals being measured, currently mice and guinea pigs. The three measurement stafions include sound and electrically shielded rooms, and specialized systems (Tucker Davis Technologies, Intelligent Hearing Systems) that include passive attenuators, filters, signal averaging algorithms, stimulus creation, etc. A fourth station will be used for generafing and controlling acousfic signals that are used for experimentally-controlled auditory damage. Our approach is to develop consistent, redundant, modular systems that provide all of these measures or subsets of these measures in a standardized, well-characterized manner;to plan for equipment and personnel redundancy so that no experiment would ever be compromised in the face ofthe usual transient breakdowns;and to develop a comprehensive server-based database so that every scientist can locate and download any result at any fime using a consistent interface.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Center Core Grants (P30)
Project #
5P30DC010363-05
Application #
8528553
Study Section
Special Emphasis Panel (ZDC1-SRB-Q)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
5
Fiscal Year
2013
Total Cost
$127,925
Indirect Cost
$47,971
Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Ealy, Megan; Ellwanger, Daniel C; Kosaric, Nina et al. (2016) Single-cell analysis delineates a trajectory toward the human early otic lineage. Proc Natl Acad Sci U S A 113:8508-13
Lee, Hee Yoon; Raphael, Patrick D; Xia, Anping et al. (2016) Two-Dimensional Cochlear Micromechanics Measured In Vivo Demonstrate Radial Tuning within the Mouse Organ of Corti. J Neurosci 36:8160-73
Zeng, Wei-Zheng; Grillet, Nicolas; Dewey, James B et al. (2016) Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function. J Neurosci 36:9201-16
Sundaresan, Srividya; Kong, Jee-Hyun; Fang, Qing et al. (2016) Thyroid hormone is required for pruning, functioning and long-term maintenance of afferent inner hair cell synapses. Eur J Neurosci 43:148-61
Sundaresan, S; Balasubbu, S; Mustapha, M (2016) Thyroid hormone is required for the pruning of afferent type II spiral ganglion neurons in the mouse cochlea. Neuroscience 312:165-78
Xia, Anping; Liu, Xiaofang; Raphael, Patrick D et al. (2016) Hair cell force generation does not amplify or tune vibrations within the chicken basilar papilla. Nat Commun 7:13133
Kim, Sangmin; Raphael, Patrick D; Oghalai, John S et al. (2016) High-speed spectral calibration by complex FIR filter in phase-sensitive optical coherence tomography. Biomed Opt Express 7:1430-44
Durruthy-Durruthy, Robert; Heller, Stefan (2015) Applications for single cell trajectory analysis in inner ear development and regeneration. Cell Tissue Res 361:49-57
Huth, Markus E; Han, Kyu-Hee; Sotoudeh, Kayvon et al. (2015) Designer aminoglycosides prevent cochlear hair cell loss and hearing loss. J Clin Invest 125:583-92
Lee, Hee Yoon; Raphael, Patrick D; Park, Jesung et al. (2015) Noninvasive in vivo imaging reveals differences between tectorial membrane and basilar membrane traveling waves in the mouse cochlea. Proc Natl Acad Sci U S A 112:3128-33

Showing the most recent 10 out of 55 publications