The Imaging Core provides access to knowledge and training allowing the user to learn and understand imaging technologies and sample preparation techniques that are tailored toward inner ear-related research. Beside standard equipment for sample preparation and microscopy, it also offers advanced imaging systems that are integrated into a physiology-oriented concept allowing access to a broad base of users of technology that is not available as off the shelf solution. The Imaging Core is integrated into the overall philosophy of the Stanford OHNS Core Center providing access to knowledge and technology (Aim 1), and to stimulate and inspire discussion among users to identify the best possible imaging solution for a specific question. Previous success supports the strategy that advanced scientific discussions foster and stimulate collaborative research (Aim 2). The core provides access and training for state-of-the-art confocal systems (Aim 3), each configured for specific tasks, such as routine confocal scanning in upright or inverted configurations, fast high resolution scanning, ultrafast scanning for imaging physiological processes in subcellular structures or spinning disk confocal imaging for living cells. In addition, users have access to a highly flexible 2-photon imaging system that allows imaging of isolated tissues and structures in living animals. For histology, the core offers access arid training to use apparatus for cryosectioning as well as paraffin embedding and sectioning.

Public Health Relevance

Access to equipment that requires expert maintenance and advanced training for optimal use of the individual instruments'capabilities can most efficiently be capitalized through the implemented Core Center. For an individual laboratory, it would be economically unbearable to maintain such broad and up-to-date capabilities. Implementing a philosophy where the user base is being educated to utilize the Core-provided technology in the best possible way creates a stimulating and innovative environment for collaborative research.

National Institute of Health (NIH)
Center Core Grants (P30)
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Special Emphasis Panel (ZDC1)
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Stanford University
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Cox, Brandon C; Chai, Renjie; Lenoir, Anne et al. (2014) Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development 141:816-29
Calton, Melissa A; Lee, Dasom; Sundaresan, Srividya et al. (2014) A lack of immune system genes causes loss in high frequency hearing but does not disrupt cochlear synapse maturation in mice. PLoS One 9:e94549
Ronaghi, Mohammad; Nasr, Marjan; Ealy, Megan et al. (2014) Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev 23:1275-84
Gao, Simon S; Wang, Rosalie; Raphael, Patrick D et al. (2014) Vibration of the organ of Corti within the cochlear apex in mice. J Neurophysiol 112:1192-204
Mendus, Diana; Sundaresan, Srividya; Grillet, Nicolas et al. (2014) Thrombospondins 1 and 2 are important for afferent synapse formation and function in the inner ear. Eur J Neurosci 39:1256-67
Durruthy-Durruthy, Robert; Gottlieb, Assaf; Hartman, Byron H et al. (2014) Reconstruction of the mouse otocyst and early neuroblast lineage at single-cell resolution. Cell 157:964-78
Aguilar, Andrea; Becker, Lars; Tedeschi, Thomas et al. (2014) ?-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion. Mol Biol Cell 25:1854-66
Guo, Zhaohua; Grimm, Christian; Becker, Lars et al. (2013) A novel ion channel formed by interaction of TRPML3 with TRPV5. PLoS One 8:e58174
Volkenstein, Stefan; Oshima, Kazuo; Sinkkonen, Saku T et al. (2013) Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus. Proc Natl Acad Sci U S A 110:14456-61
Cao, Huiren; Yin, Xiaolei; Cao, Yujie et al. (2013) FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PLoS One 8:e56516

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