The Inner Ear Physiology Core provides access to knowledge and training allowing the user to learn and understand whole animal inner ear physiology technologies as well as cellular physiology on hair cells, neurons, and heterologous expression systems. The Core is integrated into the overall philosophy of the Stanford OHNS Core Center to provide a hub for knowledge and technology (Aim 1). This includes stimulating and inspiring discussion among users and to eliminate thresholds that otherwise would prevent users to seek training to expand the scope of their work toward incorporating physiological techniques. An immediate result of such training is that is stimulates advanced discussions of scientific questions and communication among users and the Core Center personnel. The resulting stimulation of collaborative research (Aim 2) has been successful in the past funding period, which is a good indicator for the overall value of this kind of approach. The core provides access and training for measuring auditory brainstem response (ABR), distortion product otoacoustic emission (DPOAE), arid vestibular evoked potentials (VsEPs). Patch clamping and hair cell transduction experiments are provided on customized rigs and allow users to gain access to state-of-the-art technology and expertise (Aim 3) allowing them to establish experimental repertoires and research programs that without the Core Center would be impossible and economically unfeasible.
Access to equipment that requires expert maintenance and advanced training for optimal use can most efficiently be capitalized through the implemented Core Center. For an individual laboratory, it would be economically unbearable to maintain such broad and up-to-date capabilities. Implementing a philosophy where the user base is being educated to utilize the Core-provided technology in the best possible way creates a stimulating and innovative environment for collaborative research.
|Cox, Brandon C; Chai, Renjie; Lenoir, Anne et al. (2014) Spontaneous hair cell regeneration in the neonatal mouse cochlea in vivo. Development 141:816-29|
|Calton, Melissa A; Lee, Dasom; Sundaresan, Srividya et al. (2014) A lack of immune system genes causes loss in high frequency hearing but does not disrupt cochlear synapse maturation in mice. PLoS One 9:e94549|
|Ronaghi, Mohammad; Nasr, Marjan; Ealy, Megan et al. (2014) Inner ear hair cell-like cells from human embryonic stem cells. Stem Cells Dev 23:1275-84|
|Gao, Simon S; Wang, Rosalie; Raphael, Patrick D et al. (2014) Vibration of the organ of Corti within the cochlear apex in mice. J Neurophysiol 112:1192-204|
|Mendus, Diana; Sundaresan, Srividya; Grillet, Nicolas et al. (2014) Thrombospondins 1 and 2 are important for afferent synapse formation and function in the inner ear. Eur J Neurosci 39:1256-67|
|Durruthy-Durruthy, Robert; Gottlieb, Assaf; Hartman, Byron H et al. (2014) Reconstruction of the mouse otocyst and early neuroblast lineage at single-cell resolution. Cell 157:964-78|
|Aguilar, Andrea; Becker, Lars; Tedeschi, Thomas et al. (2014) ?-tubulin K40 acetylation is required for contact inhibition of proliferation and cell-substrate adhesion. Mol Biol Cell 25:1854-66|
|Guo, Zhaohua; Grimm, Christian; Becker, Lars et al. (2013) A novel ion channel formed by interaction of TRPML3 with TRPV5. PLoS One 8:e58174|
|Volkenstein, Stefan; Oshima, Kazuo; Sinkkonen, Saku T et al. (2013) Transient, afferent input-dependent, postnatal niche for neural progenitor cells in the cochlear nucleus. Proc Natl Acad Sci U S A 110:14456-61|
|Cao, Huiren; Yin, Xiaolei; Cao, Yujie et al. (2013) FCHSD1 and FCHSD2 are expressed in hair cell stereocilia and cuticular plate and regulate actin polymerization in vitro. PLoS One 8:e56516|
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